1887

Abstract

A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position. Strain M1HQ-2 grew at 4–37 °C (optimum, 30 °C), pH 5.0–11.0 (pH 8.0) and in the presence of 0–15 % (w/v) NaCl (1–3 %). Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2 belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3. Strain M1HQ-2 contained MK-7 as the dominant menaquinone. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipids profile of strain M1HQ-2 contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid. The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content of strain M1HQ-2 was 71.0 mol%. The average nucleotide identity value between strain M1HQ-2 and type strain of Brachybacterium sacelli was 76.7 %. The estimated DNA–DNA hybridization value between strain M1HQ-2 and type strain of B. sacelli was 20.6 %. On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2 represents a novel species of the genus Brachybacterium , for which the name Brachybacterium endophyticum sp. nov. is proposed. The type strain is M1HQ-2 (=KCTC 49087=CGMCC 1.16391).

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2018-09-19
2019-12-08
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References

  1. Collins MD, Brown J, Jones D. Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int J Syst Bacteriol 1988;38:45–48 [CrossRef]
    [Google Scholar]
  2. Gvozdyak OR, Nogina TM, Schumann P. Taxonomic study of the genus Brachybacterium: Brachybacterium nesterenkovii sp. nov. Int J Syst Bacteriol 1992;42:74–78 [CrossRef][PubMed]
    [Google Scholar]
  3. Takeuchi M, Fang C-X, Yokota A. Taxonomic study of the genus Brachybacterium: proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov. Int J Syst Bacteriol 1995;45:160–168 [CrossRef]
    [Google Scholar]
  4. Schubert K, Ludwig W, Springer N, Kroppenstedt RM, Accolas JP et al. Two coryneform bacteria isolated from the surface of french gruyère and beaufort cheeses are new species of the genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov.r. Int J Syst Evol Microbiol 1996;45:81–87
    [Google Scholar]
  5. Heyrman J, Balcaen A, de Vos P, Schumann P, Swings J et al. Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Evol Microbiol 2002;52:1641–1646 [CrossRef][PubMed]
    [Google Scholar]
  6. Buczolits S, Schumann P, Weidler G, Radax C, Busse HJ et al. Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int J Syst Evol Microbiol 2003;53:1955–1960 [CrossRef][PubMed]
    [Google Scholar]
  7. Zhang G, Zeng G, Cai X, Deng S, Luo H et al. Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China. Int J Syst Evol Microbiol 2007;57:2519–2524 [CrossRef][PubMed]
    [Google Scholar]
  8. Chou JH, Lin KY, Lin MC, Sheu SY, Wei YH et al. Brachybacterium phenoliresistens sp. nov., isolated from oil-contaminated coastal sand. Int J Syst Evol Microbiol 2007;57:2674–2679 [CrossRef][PubMed]
    [Google Scholar]
  9. Gontia I, Kavita K, Schmid M, Hartmann A, Jha B et al. Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential. Int J Syst Evol Microbiol 2011;61:2799–2804 [CrossRef][PubMed]
    [Google Scholar]
  10. Park SK, Kim MS, Jung MJ, Nam YD, Park EJ et al. Brachybacterium squillarum sp. nov., isolated from salt-fermented seafood. Int J Syst Evol Microbiol 2011;61:1118–1122 [CrossRef][PubMed]
    [Google Scholar]
  11. Hoang VA, Kim YJ, Nguyen NL, Yang DC. Brachybacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2014;64:3063–3068 [CrossRef][PubMed]
    [Google Scholar]
  12. Liu Y, Xie QY, Shi W, Li L, An JY et al. Brachybacterium huguangmaarense sp. nov., isolated from Lake sediment. Int J Syst Evol Microbiol 2014;64:1673–1678 [CrossRef][PubMed]
    [Google Scholar]
  13. Liu Y, Zhai L, Yao S, Cao Y, Cao Y et al. Brachybacterium hainanense sp. nov., isolated from noni (Morinda citrifolia L.) branch. Int J Syst Evol Microbiol 2015;65:4196–4201 [CrossRef][PubMed]
    [Google Scholar]
  14. Kaur G, Kumar N, Mual P, Kumar A, Kumar RM et al. Brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater. Int J Syst Evol Microbiol 2016;66:4705–4710 [CrossRef][PubMed]
    [Google Scholar]
  15. Singh H, Du J, Yang JE, Shik Yin C, Kook M et al. Brachybacterium horti sp. nov., isolated from garden soil. Int J Syst Evol Microbiol 2016;66:189–195 [CrossRef][PubMed]
    [Google Scholar]
  16. Qin S, Wang HB, Chen HH, Zhang YQ, Jiang CL et al. Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 2008;58:2525–2528 [CrossRef][PubMed]
    [Google Scholar]
  17. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007;57:1424–1428 [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  19. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  20. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  21. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  22. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  23. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  25. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009;106:19126–19131 [CrossRef][PubMed]
    [Google Scholar]
  26. Magee CM, Rodeheaver G, Edgerton MT, Edlich RF. A more reliable gram staining technic for diagnosis of surgical infections. Am J Surg 1975;130:341–346 [CrossRef][PubMed]
    [Google Scholar]
  27. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  28. Kelly KL. Inter-Society Color Council-National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
    [Google Scholar]
  29. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005;55:1149–1153 [CrossRef][PubMed]
    [Google Scholar]
  30. Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual, 6th ed. San Francisco: Benjamin Cummings Pearson Education; 2002
    [Google Scholar]
  31. Gonzalez C, Gutierrez C, Ramirez C. Halobacterium vallismortis sp. nov. an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 1978;24:710–715 [CrossRef][PubMed]
    [Google Scholar]
  32. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972;36:407–477[PubMed]
    [Google Scholar]
  33. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  34. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977;100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  35. Guo L, Tuo L, Habden X, Zhang Y, Liu J et al. Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. Int J Syst Evol Microbiol 2015;65:206–213 [CrossRef][PubMed]
    [Google Scholar]
  36. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI inc; 1990
    [Google Scholar]
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