1887

Abstract

A pale yellow bacterial strain, designated SDU1-6, was isolated from a soil sample collected in the Jinan campus of Shandong University, Shandong Province, PR China. The strain was aerobic, motile, Gram-stain-negative and rod-shaped. Cellular growth occurred at 13–37 °C, pH 5.0–10.0 and with 0–0.1 % (w/v) NaCl, and the optimal growth was at 25 °C and pH 7.5. SDU1-6 had the highest 16S rRNA gene similarity of 95.42 % with Chryseolinea serpens DSM 24574. The genome was 6 542 746 bp in length with 43.5 mol% DNA G+C content. The major fatty acids of SDU1-6 were C16 : 1ω5 and iso-C15 : 0, the major menaquinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. On the basis of the polyphasic evidence and the 16S rRNA gene sequence, SDU1-6 represents a novel species of the genus Chryseolinea , for which the name Chryseolinea flava sp. nov. is proposed. The type strain is SDU1-6 (=CGMCC 1.13492=JCM 32520).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.003022
2018-09-17
2024-10-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/11/3518.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.003022&mimeType=html&fmt=ahah

References

  1. Kim JJ, Alkawally M, Brady AL, Rijpstra WI, Sinninghe Damsté JS et al. Chryseolinea serpens gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from soil. Int J Syst Evol Microbiol 2013; 63:654–660 [View Article][PubMed]
    [Google Scholar]
  2. Reichenbach H, Dworkin M. The myxobacteria. In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH et al. (editors) The Prokaryotes, 2nd ed. New York: Springer-Verlag; 1992 pp. 3416–3487
    [Google Scholar]
  3. Dong XZ, Cai MY. (editors) Chapter 12. Morphological characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp. 370–398
    [Google Scholar]
  4. Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
    [Google Scholar]
  5. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  6. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101:11030–11035 [View Article][PubMed]
    [Google Scholar]
  7. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  8. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
    [Google Scholar]
  10. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article][PubMed]
    [Google Scholar]
  11. Breznak JA, Costilow RN. Physicochemical factors in growth. In Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR et al. (editors) Methods for General and Molecular bacteriology Washington, DC: American Society for Microbiology; 2007 pp. 309–329
    [Google Scholar]
  12. Han JR, Zhang H, Zheng WS, Chen GJ, Du ZJ. Confluentibacter citreus sp. nov., isolated from lake sediment, and emended description of the genus Confluentibacter. Int J Syst Evol Microbiol 2017; 67:4008–4012 [View Article][PubMed]
    [Google Scholar]
  13. Claus D. A standardized Gram staining procedure. World J Microbiol Biotechnol 1992; 8:451–452 [View Article][PubMed]
    [Google Scholar]
  14. Dong XZ, Cai MY. (editors) Chapter 14. Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp. 370–398
    [Google Scholar]
  15. Yan L, Wang J, Chen Z, Guan Y, Li J. Microbacterium nanhaiense sp. nov., an actinobacterium isolated from sea sediment. Int J Syst Evol Microbiol 2015; 65:3697–3702 [View Article][PubMed]
    [Google Scholar]
  16. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  17. da Costa MS, Albuquerque L, Nobre MF, Wait R. The identification of fatty acids in bacteria. In Rainey FA, Oren A. (editors) Methods in Microbiology (Taxonomy of Prokaryotes) vol. 38 London: Elsevier Ltd; 2011 pp. 183–196
    [Google Scholar]
  18. Sabry SA, Ghanem NB, Abu-Ella GA, Schumann P, Stackebrandt E et al. Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 2004; 54:453–456 [View Article][PubMed]
    [Google Scholar]
  19. da Costa MS, Albuquerque L, Nobre MF, Wait R. The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. In Rainey FA, Oren A. (editors) Methods in Microbiology (Taxonomy of Prokaryotex) London: Elsevier Ltd; 2011 pp. 197–206
    [Google Scholar]
  20. Tindall BJ, Sikorski J, Smibert RM, Kreig NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007 pp. 330–393
    [Google Scholar]
  21. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.003022
Loading
/content/journal/ijsem/10.1099/ijsem.0.003022
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error