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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 68, Issue 11

Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses Free

Abstract

Three strains, JCM 5343, JCM 5344 and JCM 1130, currently identified as Lactobacillus gasseri , were investigated using a polyphasic taxonomic approach. Although these strains shared high 16S rRNA gene sequence similarities with L. gasseri ATCC 33323 (99.9 %), they formed a clade clearly distinct from ATCC 33323 based on whole-genome relatedness. The average nucleotide identity and in silico DNA–DNA hybridization values of these three strains compared to L. gasseri ATCC 33323 were 93.4–93.7 and 53.1–54.1 %, respectively, and both were less than the widely accepted threshold to distinguish two species (95 and 70 %, respectively). The three strains were Gram-stain positive, non-motile, non-spore-forming, catalase-negative and rod-shaped bacteria. They grew at 25–45 °C and in the presence of 2.0 % (w/v) NaCl. The major fatty acids of the three strains were C16 : 0 and C18 : 1 ω9c. Based on phylogenetic analyses of the phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit genes, and on phenotypic and chemotaxonomic characteristics, strains JCM 5343, JCM 5344 and JCM 1130 represent a novel species distinct from L. gasseri , for which we propose the name Lactobacillus paragasseri sp. nov. In addition, a large portion of genomes currently labelled as L. gasseri in the public sequence database should be reclassified as L. paragasseri based on whole-genome relatedness.

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Keyword(s): Lactobacillus gasseri and Lactobacillus paragasseri
© 2018 IUMS
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2018-09-18
2025-01-22
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/content/journal/ijsem/10.1099/ijsem.0.003020
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Lactobacillus paragasseri sp. nov., a sister taxon of Lactobacillus gasseri, based on whole-genome sequence analyses
Int J Syst Evol Microbiol 68, 3512 (2018); https://doi.org/10.1099/ijsem.0.003020
/content/journal/ijsem/10.1099/ijsem.0.003020
/content/journal/ijsem/10.1099/ijsem.0.003020
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