1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 11

Rubrobacter indicoceani sp. nov., a new marine actinobacterium isolated from Indian Ocean sediment Free

Abstract

A novel mesophilic marine actinobacterial strain, designated as SCSIO 08198, was isolated from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-positive, rod-shaped and salmon pink in colour. Good growth occurred on marine agar with 1–5 % (w/v) NaCl and incubation at 28 °C for more than a fortnight. Sensitive to short ultraviolet radiation. Analysis of 16S rRNA gene sequences revealed that strain SCSIO 08198 had the highest similarity of 97.2 % to Rubrobacter radiotolerans DSM 5868, and loosely related (<94.2 %) to all other species in the genus Rubrobacter . Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genus Rubrobacter . The total cellular fatty acid profile was dominated by C16 : 0 12-methyl. MK-8 was the main menaquinone. The peptidoglycan type was A3α (l-Lys-l-Ala). The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol and unidentified phospholipids. Based on the whole genome sequence analysis, the genome size is 3 078 689 bp with DNA G+C value of 63.8 mol%, including one circular chromosome and two plasmids. Based on these polyphasic data, a new species, Rubrobacter indicoceani sp. nov., is proposed, with the type strain SCSIO 08198 (=DSM 105148=CGMCC 1.16398).

  • Received:
  • Accepted:
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Keyword(s): Indian Ocean , marine actinobacteria , polyphasic taxonomy , Rubrobacter and Rubrobacter indicoceani sp. nov.
© 2018 IUMS
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2018-10-09
2024-04-23
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Rubrobacter indicoceani sp. nov., a new marine actinobacterium isolated from Indian Ocean sediment
Int J Syst Evol Microbiol 68, 3487 (2018); https://doi.org/10.1099/ijsem.0.003018
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