1887

Abstract

A novel basophilic bacterial strain, designated as SCSIO 08040, was recovered from a deep-sea sediment sample collected from the Indian Ocean. The strain was Gram-stain-negative, vibrioid or spiral, light pink, 0.6–1.0 µm wide and 1.0–2.5 µm long. Growth occurred at 20–45 °C, pH 7–11 and <5 % (w/v) NaCl, with optimum growth at 28–37 °C, pH 7 and 0–3 % (w/v) NaCl. Catalase-, oxidase and urease-positive, nitrate reduction-negative. Analysis of 16S rRNA gene sequencing revealed that strain SCSIO 08040 had the highest similarity of 95.3 % to Rhodocista pekingensis 3-p. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences showed that the novel isolate formed a distinct phylogenetic lineage in the family Rhodospirillaceae . The whole-cell hydrolysate contained meso–diaminopimelic acid, galactose, mannose and xylose. The total cellular fatty acid profile was dominated by C18:1ω7c and C19:0cycloω8c. Q-10 was the predominant ubiquinone. The major phospholipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The DNA G+C content of strain SCSIO 08040 was 66.82 mol%. Based on these polyphasic data, a new genus, Indioceanicola gen. nov., is proposed in the family Rhodospirillaceae with the type species Indioceanicola profundi sp. nov. and the type strain SCSIO 08040 (=DSM 105146=CGMCC 1.15812).

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2018-10-11
2019-09-22
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