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Volume 68, Issue 10

Streptomyces venetus sp. nov., an actinomycete with a blue aerial mycelium Free

Abstract

A Gram-positive bacterium, designated CMU-AB225, was isolated from rhizosphere soil of an oil palm (Elaeis guineensis). The strain exhibited a blue aerial spore mass and a light cream to moderate yellow substrate mycelium and formed chains of spiny spores. Whole-cell hydrolysates consisted of ll-diaminopimelic acid, glucose, ribose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The polar lipids profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside, four unidentified lipids, two unidentified aminolipids and an unidentified glycolipid. The major cellular fatty acids (>10 %) were iso-C16 : 0, C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The G+C content of genomic DNA was 69.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMU-AB225 was a member of the genus Streptomyces and formed a distinct phyletic line which was most closely related to Streptomyces koyangensis JCM 14915, Streptomyces misionensis JCM 4497 and Streptomyces aurantiogriseus JCM 4346. Multilocus sequence analysis (MLSA) using five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA distances of strain CMU-AB225 to phylogenetically related species were greater than the 0.007 threshold. Moreover, the low values of DNA–DNA relatedness and phenotypic differences, especially a blue aerial mycelium, enabled strain CMU-AB225 to be distinguished from its closely related species. It is thus proposed that strain CMU-AB225 represents a novel species, namely Streptomyces venetus sp. nov. The type strain is CMU-AB225 (=JCM 31290=TBRC 2001).

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  • Accepted:
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Keyword(s): blue aerial mycelium , multilocus sequence analysis , oil palm , rhizosphere soil and Streptomyces
© 2018 IUMS
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2018-08-31
2024-04-25
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Streptomyces venetus sp. nov., an actinomycete with a blue aerial mycelium
Int J Syst Evol Microbiol 68, 3333 (2018); https://doi.org/10.1099/ijsem.0.002995
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