1887

Abstract

A novel actinomycete, designated strain XZHG99, was isolated from soil taken from colour desert, Dengpa District, Tibet Autonomous Region, China. Its taxonomic position was determined by a polyphasic approach. The strain showed morphological and chemotaxonomic features typical of the genus Streptomyces : branched substrate and aerial mycelia, straight spore chains and smooth spores; ll-diaminopimelic acid in the cell-wall peptidoglycan; MK-9(H8), MK-9(H2), MK-9(H6) and MK-9(H10) as menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylinositol, phosphotidylinositol mannoside and phosphatidylglycerol as prominent phospholipids; iso-C16 : 0, anteiso-C15 : 0 and C16 : 0 as major cellular fatty acids; and DNA G+C content of 69.9 mol%. 16S rRNA gene sequence analysis indicated that strain XZHG99 showed high similarity to Streptomyces albiflavescens m20 (98.42 %) and Streptomyces krungchingensis KC-035 (98.14 %) as well as formed a monophyletic clade with them in the phylogenetic tree. Based on comparison of phenotypic properties and the low level of DNA–DNA relatedness, strain XZHG99 can be distinguished from phylogenetically related Streptomyces species and is suggested to represent a novel species of the genus Streptomyces , for which the name Streptomyces dengpaensis sp. nov. is proposed. The type strain is XZHG99 (=CGMCC 4.7472=KCTC 49090).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002994
2018-08-31
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/10/3322.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002994&mimeType=html&fmt=ahah

References

  1. Hopwood DA. Streptomyces in Nature and Medicine: The Antibiotic Makers NY: Oxford University; 2007
    [Google Scholar]
  2. Barka EA, Vatsa P, Sanchez L, Gaveau-Vaillant N, Jacquard C et al. Taxonomy, physiology, and natural products of actinobacteria. Microbiol Mol Biol Rev 2016;80:1–43 [CrossRef][PubMed]
    [Google Scholar]
  3. Thumar JT, Dhulia K, Singh SP. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis strain Kut-8. World J Microbiol Biotechnol 2010;26:2081–2087 [CrossRef]
    [Google Scholar]
  4. Santhanam R, Okoro CK, Rong X, Huang Y, Bull AT et al. Streptomyces atacamensis sp. nov., isolated from an extreme hyper-arid soil of the Atacama desert, Chile. Int J Syst Evol Microbiol 2012;62:2680–2684 [CrossRef][PubMed]
    [Google Scholar]
  5. Santhanam R, Rong X, Huang Y, Andrews BA, Asenjo JA et al. Streptomyces bullii sp. nov., isolated from a hyper-arid Atacama Desert soil. Antonie van Leeuwenhoek 2013;103:367–373 [CrossRef][PubMed]
    [Google Scholar]
  6. Schulz D, Beese P, Ohlendorf B, Erhard A, Zinecker H et al. Abenquines A-D: aminoquinone derivatives produced by Streptomyces sp. strain DB634. J Antibiot 2011;64:763–768 [CrossRef][PubMed]
    [Google Scholar]
  7. Hayakawa M. Studies on the isolation and distribution of rare actinomycetes in soil. Actinomycetologica 2008;22:12–19 [CrossRef]
    [Google Scholar]
  8. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  9. Kelly KL. Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
    [Google Scholar]
  10. Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 1969;19:391–512 [CrossRef]
    [Google Scholar]
  11. Collee JG, Miles RS, Watt B. Tests for identification of bacteria. In Collee JG, Fraser AG, Marmion BP, Simmons A. (editors) Practical Medical Microbiology, 14th ed. New York: Churchill Livingstone; 1996; pp.131–149
    [Google Scholar]
  12. Arai T. Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan; 1975
    [Google Scholar]
  13. Williams ST, Cross T. Actinomycetes. Methods Microbiol 1971;4:295–334
    [Google Scholar]
  14. Hsu SC, Lockwood JL. Powdered chitin agar as selective medium for enumeration of actinomycetes in water and soil. Appl Microbial 2007;29:422–426
    [Google Scholar]
  15. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  16. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983;129:1743–1813 [CrossRef][PubMed]
    [Google Scholar]
  17. Goodfellow M. Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 1971;69:33–80 [CrossRef][PubMed]
    [Google Scholar]
  18. Hugo WB, Russell AD. Pharmaceutical Microbiology, 6th ed. Oxford: Blackwell; 1998
    [Google Scholar]
  19. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970;20:435–443 [CrossRef]
    [Google Scholar]
  20. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29:319–322 [CrossRef]
    [Google Scholar]
  21. Collins MD, Howarth OW, Grund E, Kroppenstedt RM. Isolation and structural determination of new members of the vitamin K2 series in Nocardia brasiliensis. FEMS Microbiol Lett 1987;41:35–39 [CrossRef]
    [Google Scholar]
  22. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987;19:61–206
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark DE: MIDI Inc; 1990
    [Google Scholar]
  24. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996;42:989–1005 [CrossRef]
    [Google Scholar]
  25. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  26. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983;4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  27. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  28. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  29. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  30. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  31. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  32. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  33. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006;33:152–155
    [Google Scholar]
  34. Wayne IG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. In J Syst Bacteriol 1987;463–464
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002994
Loading
/content/journal/ijsem/10.1099/ijsem.0.002994
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error