1887

Abstract

Two closely related isolates, 27335 and 24999, of rapidly growing, non-pigmented mycobacteria, were cultured from two clinically ill fish of the family Syngnathidae. Whole genome sequencing of the two isolates revealed low sequence homology to documented mycobacteria within public databases such as the NCBI. Evaluation of targeted housekeeping genes, including 16S rRNA, ITS, rpoB and hsp65, related the two bacteria distantly to Mycobacterium senegalense CK2 M4421 and Mycobacterium farcinogenes DSM 43637. Phenotypic, biochemical and dDNA–DNA hybridization tests demonstrated that Mycobacterium syngnathidarum is a new species distinct from other recognized rapidly growing mycobacterial species. Phenotypic, chemotaxonomic and phylogenetic data evaluation provided evidence that the two strains represent one novel species. We propose the formal recognition of Mycobacterium syngnathidarum sp. nov., with isolate 27335 as the type strain (=ATCC TSD-89,=DSM 105112).

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2018-10-01
2019-10-19
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References

  1. Qvist T, Gilljam M, Jönsson B, Taylor-Robinson D, Jensen-Fangel S et al. Epidemiology of nontuberculous mycobacteria among patients with cystic fibrosis in Scandinavia. J Cyst Fibros 2015;14:46–52 [CrossRef][PubMed]
    [Google Scholar]
  2. Mayskaya MU, Otten TF, Ariel BM, Fedotova EP, Hunter RL et al. Morphological manifestations of the atypical mycobacteriosis caused by nontuberculous mycobacteria in the HIV infected patients. Ann Clin Lab Sci 2014;44:131–133[PubMed]
    [Google Scholar]
  3. Tanagho A, Hatab S, Hawkins A. Atypical osteomyelitis caused by Mycobacterium chelonae in a nonimmunocompromised patient: a case report. JBJS Case Connect 2015;5:e17 [CrossRef][PubMed]
    [Google Scholar]
  4. Lickiss J, Olsen A, Ryan JD. Mycobacterium chelonae-abscessus complex infection after flatfoot reconstruction. J Foot Ankle Surg 2016;55:1327–1332 [CrossRef][PubMed]
    [Google Scholar]
  5. Decostere A, Hermans K, Haesebrouck F. Piscine mycobacteriosis: a literature review covering the agent and the disease it causes in fish and humans. Vet Microbiol 2004;99:159–166 [CrossRef][PubMed]
    [Google Scholar]
  6. Johnson MM, Odell JA. Nontuberculous mycobacterial pulmonary infections. J Thorac Dis 2014;6:210 [CrossRef][PubMed]
    [Google Scholar]
  7. Lai CC, Tan CK, Chou CH, Hsu HL, Liao CH et al. Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000–2008. Emerg Infect Dis 2010;16:294–296 [CrossRef][PubMed]
    [Google Scholar]
  8. Bar-On O, Mussaffi H, Mei-Zahav M, Prais D, Steuer G et al. Increasing nontuberculous mycobacteria infection in cystic fibrosis. J Cyst Fibros 2015;14:53–62 [CrossRef][PubMed]
    [Google Scholar]
  9. Slany M, Jezek P, Fiserova V, Bodnarova M, Stork J et al. Mycobacterium marinum infections in humans and tracing of its possible environmental sources. Can J Microbiol 2012;58:39–44 [CrossRef][PubMed]
    [Google Scholar]
  10. Falkinham JO. Nontuberculous mycobacteria from household plumbing of patients with nontuberculous mycobacteria disease. Emerg Infect Dis 2011;17:419–424 [CrossRef][PubMed]
    [Google Scholar]
  11. Yanong RP, Pouder DB, Falkinham JO. Association of mycobacteria in recirculating aquaculture systems and mycobacterial disease in fish. J Aquat Anim Health 2010;22:219–223 [CrossRef][PubMed]
    [Google Scholar]
  12. Gomez-Alvarez V, Revetta RP. Whole-genome sequences of four strains closely related to members of the Mycobacterium chelonae group, isolated from biofilms in a drinking water distribution system simulator. Genome Announc 2016;4:e0153901515 [CrossRef][PubMed]
    [Google Scholar]
  13. Feazel LM, Baumgartner LK, Peterson KL, Frank DN, Harris JK et al. Opportunistic pathogens enriched in showerhead biofilms. Proc Natl Acad Sci USA 2009;106:16393–16399 [CrossRef][PubMed]
    [Google Scholar]
  14. Le Dantec C, Duguet JP, Montiel A, Dumoutier N, Dubrou S et al. Chlorine disinfection of atypical mycobacteria isolated from a water distribution system. Appl Environ Microbiol 2002;68:1025–1032 [CrossRef][PubMed]
    [Google Scholar]
  15. Falkinham JO. Environmental sources of nontuberculous mycobacteria. Clin Chest Med 2015;36:35–41 [CrossRef][PubMed]
    [Google Scholar]
  16. Kent PT, Kubica GP. Public Health Mycobacteriology: A Guide for the Level III Laboratory US department of health and human services, public health service, Centers for Disease Control 1985
    [Google Scholar]
  17. Cavusoglu C, Gurpinar T, Ecemis T. Evaluation of antimicrobial susceptibilities of rapidly growing mycobacteria by Sensititre RAPMYCO panel. New Microbiol 2012;35:73–76[PubMed]
    [Google Scholar]
  18. Woods GL, Brown-Elliott B, Desmond EP, Hall GS, Heifets L et al. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes: approved standard: NCCLS. 2003
  19. CLSI Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomyces; Approved Standard, 2nd ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2011
    [Google Scholar]
  20. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 2014;30:2114–2120 [CrossRef][PubMed]
    [Google Scholar]
  21. Nurk S, Bankevich A, Antipov D, Gurevich A, Korobeynikov A et al. Assembling genomes and mini-metagenomes from highly chimeric reads. In Deng M, Jiang R, Sun F, Zhang X. (editors) Research in Computational Molecular Biology: 17th Annual International Conference, RECOMB 2013, Beijing, China, April 7-10, 2013. Proceedingsvol. 2013 Berlin, Heidelberg: Springer Berlin Heidelberg; pp.158–170
    [Google Scholar]
  22. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013;29:1072–1075 [CrossRef][PubMed]
    [Google Scholar]
  23. Aziz RK, Bartels D, Best AA, Dejongh M, Disz T et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 2008;9:75 [CrossRef][PubMed]
    [Google Scholar]
  24. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M et al. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 2012;28:1647–1649 [CrossRef][PubMed]
    [Google Scholar]
  25. Adékambi T, Colson P, Drancourt M. rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 2003;41:5699–5708 [CrossRef][PubMed]
    [Google Scholar]
  26. Adékambi T, Drancourt M. Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int J Syst Evol Microbiol 2004;54:2095–2105 [CrossRef][PubMed]
    [Google Scholar]
  27. Telenti A, Marchesi F, Balz M, Bally F, Böttger EC et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175–178[PubMed]
    [Google Scholar]
  28. Roth A, Reischl U, Streubel A, Naumann L, Kroppenstedt RM et al. Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases. J Clin Microbiol 2000;38:1094–1104[PubMed]
    [Google Scholar]
  29. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004;32:1792–1797 [CrossRef][PubMed]
    [Google Scholar]
  30. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014;30:1312–1313 [CrossRef][PubMed]
    [Google Scholar]
  31. Meier-Kolthoff JP, Klenk HP, Göker M. Taxonomic use of DNA G+C content and DNA-DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014;64:352–356 [CrossRef][PubMed]
    [Google Scholar]
  32. Auch AF, von Jan M, Klenk HP, Göker M. Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef][PubMed]
    [Google Scholar]
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