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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 10

Lactobacilus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter Free

Abstract

A rod-shaped, Gram-stain-positive, non-flagellated, facultatively anaerobic bacterium, designated SYF10-1a, was isolated from Nuruk, a Korean traditional fermentation starter. It grew at 4–40 °C (optimum 37 °C), at pH 3.0–9.0 (optimum, pH 7.0) and with 0–5 % (w/v) NaCl. Phylogenetic analysis using 16S rRNA gene sequences revealed that strain SYF10-1a belonged to the genus Lactobacillus and showed the highest sequence similarity of 98.7 % to Lactobacillus crustorum LMG 23699. A comparison of two housekeeping genes, pheS and rpoA, supported the suggestion that strain SYF10-1a fell within the radius of the genus Lactobacillus , but was clearly separated from its closest related species. The average nucleotide identity value and digital DNA–DNA hybridization value between strain SYF10-1a and the most closely related species, L. crustorum LMG 23699, were 80.5 and 33.3 %, respectively. The predominant cellular fatty acids were C16 : 0, C18 : 1ω9c, C18 : 1ω7c and summed feature 3 (including iso-C15 : 0 2-OH and/or C16 : 1ω7c). Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminolipids and two unidentified glycolipids. The cell-wall peptidoglycan was of the A4α type with an interpeptide bridge comprising l-Lys-d-Asp. The DNA G+C content was 34.2 mol%. On the basis of this taxonomic study, strain SYF10-1a represents a novel species within the genus Lactobacillus , for which the name Lactobacillus nuruki sp. nov. is proposed. The type strain is SYF10-1a (=KACC 18726=NBRC 112011).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Firmicutes , Lactobacillus nuruki and Nuruk
© 2018 IUMS
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2018-08-29
2024-12-03
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Lactobacilus nuruki sp. nov., isolated from Nuruk, a Korean fermentation starter
Int J Syst Evol Microbiol 68, 3273 (2018); https://doi.org/10.1099/ijsem.0.002976
/content/journal/ijsem/10.1099/ijsem.0.002976
/content/journal/ijsem/10.1099/ijsem.0.002976
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