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Volume 68, Issue 10

Paenibacillus xerothermodurans sp. nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida Free

Abstract

A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA. Growth was observed at 20–42 °C (optimum, 37 °C), at pH 6.0–10.0 (optimum pH 7.0) and in the presence of 0.5–3 % NaCl (optimum 0.5 %). The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7. The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid. The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0. Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380 to the genus Paenibacillus , and showed the highest sequence similarity to Paenibacillus rigui JCM 16352 (97.0 %). The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %. The draft genome of ATCC 27380 had a size of 4,361,187 bases, with a G+C content of 51.0 %. The average nucleotide identity and in silico DNA–DNA hybridization values between strain ATCC 27380 and P. rigui JCM 16352 were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively). The average amino acid identity between strain ATCC 27380 and P. rigui JCM 16352 was 68.72 %, which was above the suggested genus level demarcation of 65 %. Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380 represents a novel species in the genus Paenibacillus , for which the name Paenibacillus xerothermodurans sp. nov. (=DSM 520=NRRL NRS-1629=ATCC 27380) is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): dry heat resistant , novel species and Paenibacillus xerothermodurans
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2018-08-21
2024-04-20
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Paenibacillus xerothermodurans sp. nov., an extremely dry heat resistant spore forming bacterium isolated from the soil of Cape Canaveral, Florida
Int J Syst Evol Microbiol 68, 3190 (2018); https://doi.org/10.1099/ijsem.0.002967
/content/journal/ijsem/10.1099/ijsem.0.002967
/content/journal/ijsem/10.1099/ijsem.0.002967
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