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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 68, Issue 10

Primorskyibacter marinus sp. nov., isolated from coastal sediment Free

Abstract

A novel Gram-stain-negative, whitish-yellow, rod-shaped, non-pigmented, aerobic, oxidase-positive and catalase-positive bacterium, designated PX7, was isolated from coastal sediment of Xiaoshi Island, Weihai, China (37° 31′ 36″ N, 122° 00′ 58″ E). Strain PX7 grew optimally at 30 °C, at pH 7.0–7.5 and in the presence of 3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PX7 formed a robust clade with members of the genus Primorskyibacter and was closely related to Primorskyibacter sedentarius , Primorskyibacter aestuariivivens and Primorskyibacter insulae with 96.5, 96.2 and 95.1 % sequence similarities, respectively. The sole respiratory quinone of strain PX7 was ubiquinone-10, and the dominant fatty acid was C18 : 1ω7c (80.2 %). The major polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and one unidentified lipid. The DNA G+C content of strain PX7 was 60.2 mol%. Based on the combination of phylogenetic analyses, phenotypic and chemotaxonomic data, strain PX7 is considered to represent a novel species within the genus Primorskyibacter in the family Rhodobacteraceae , for which the name Primorskyibacter marinus sp. nov. is proposed. The type strain of the new species is PX7 (=KCTC 42952=MCCC 1H00196).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Polyphasic taxonomy , Primorskyibacter and Rhodobacteraceae
© 2018 IUMS
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2018-08-09
2025-01-24
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References

  1. Romanenko LA, Tanaka N, Svetashev VI, Mikhailov VV. Primorskyibacter sedentarius gen. nov., sp. nov., a novel member of the class Alphaproteobacteria from shallow marine sediments. Int J Syst Evol Microbiol 2011; 61:1572–1578 [View Article][PubMed]
     [Google Scholar]
  2. Park S, Yoon SY, Ha MJ, Jung YT, Yoon JH. Primorskyibacter aestuariivivens sp. nov., isolated from a tidal flat, and emended description of the genus Primorskyibacter. Int J Syst Evol Microbiol 2016; 66:5550–5555 [View Article][PubMed]
     [Google Scholar]
  3. Park S, Park JM, Jung YT, Won SM, Yoon JH. Primorskyibacter insulae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 2015; 65:3971–3976 [View Article][PubMed]
     [Google Scholar]
  4. Arahal DR, Macián MC, Garay E, Pujalte MJ. Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 2005; 55:2371–2376 [View Article][PubMed]
     [Google Scholar]
  5. Lee OO, Tsoi MM, Li X, Wong PK, Qian PY. Thalassococcus halodurans gen. nov., sp. nov., a novel halotolerant member of the Roseobacter clade isolated from the marine sponge Halichondria panicea at Friday Harbor, USA. Int J Syst Evol Microbiol 2007; 57:1919–1924 [View Article][PubMed]
     [Google Scholar]
  6. Liu QQ, Xl L, Rooney AP, Zj D, Chen GJ et al. Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. Int J Syst Evol Microbiol 2014; 64:3473–3477
     [Google Scholar]
  7. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  8. Guo LY, Dunlap CA, Rooney AP, Chen GJ, Du ZJ. Wenzhouxiangella sediminis sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 2016; 66:4575–4579 [View Article][PubMed]
     [Google Scholar]
  9. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  10. Han JR, Fang DB, Xia HF, Chen GJ, Du ZJ. Aquimarina rubra sp. nov., isolated from sediment of a sea cucumber culture pond. Int J Syst Evol Microbiol 2017; 67:1932–1936 [View Article][PubMed]
     [Google Scholar]
  11. Dong XZ, Cai MY. Chapter 14. Determination of biochemical characteristics. In Manual for the Systematic Identification of General Bacteria Beijing: Science Press; 2001 pp. 370–398
     [Google Scholar]
  12. Wang B, Tan T, Shao Z. Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 2009; 59:1116–1121 [View Article][PubMed]
     [Google Scholar]
  13. Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ. Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 2014; 64:1690–1696 [View Article][PubMed]
     [Google Scholar]
  14. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  15. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42:457–469 [View Article]
     [Google Scholar]
  16. Xu XW, Wu YH, Wang CS, Oren A, Zhou PJ et al. Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 2007; 57:717–720 [View Article][PubMed]
     [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  18. Stackebrandt E, Goebel BM. Taxonomic Note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
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Primorskyibacter marinus sp. nov., isolated from coastal sediment
Int J Syst Evol Microbiol 68, 3169 (2018); https://doi.org/10.1099/ijsem.0.002959
/content/journal/ijsem/10.1099/ijsem.0.002959
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