1887

Abstract

A moderately acidophilic actinobacterial strain, designated MMS16-CNU450, was isolated from pine grove soil, and its taxonomic position was analysed using a polyphasic approach. The isolate showed best growth at 30 °C, pH 6 and 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence similarity, the isolate was assigned to the genus Streptacidiphilus , and the closest species were Streptacidiphilus rugosus AM-16 (sequence similarity, 98.61 %), Streptacidiphilus melanogenes NBRC 103184 (98.53 %), Streptacidiphilus jiangxiensis NBRC 100920 (98.19 %) and Streptacidiphilus anmyonensis NBRC 103185 (98.05 %). The isolate formed a distinct cluster of its own within the Streptacidiphilus clade in the phylogenetic tree. Based on whole-genome comparison between the strain MMS16-CNU450 and the type strains of related species, the orthologous average nucleotide identity and in silico DNA–DNA hybridization values were in the range of 77.9–87.0 and 22.3–32.7 %, respectively. The DNA G+C content of the isolate was 68.6 mol%. The phylogenetic, phenotypic, chemotaxonomic and genomic data supported the affiliation of the strain to Streptacidiphilus , and the name Streptacidiphilus pinicola sp. nov. (type strain, MMS16-CNU450=KCTC 49008=JCM 32300) is proposed accordingly.

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2018-08-13
2019-09-17
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