1887

Abstract

A Gram-negative, strictly aerobic, non-motile and short rod- or coccus-shaped bacterium, designated strain LYH11, was isolated from a freshwater green alga Paulinella chromatophora. The strain grew at 5–37 °C (optimum, 30 °C) and pH 6–9 (pH 7) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LYH11 clearly belonged to the genus Kaistia of the family Rhizobiaceae . Strain LYH11 shared the highest 16S rRNA gene sequence similarity to Kaistia soli 5YN9-8 (98.3 %), Kaistia terrae 5YN3-3 (98.2 %), Kaistia geumhonensis B1-1 (97.8 %), Kaistia defluvii B6-12 (97.4 %) and Kaistia granuli Ko04 (97.2 %). The average nucleotide identity and in silico DNA–DNA hybridization values between strain LYH11 and K. soli 5YN9-8, the closest Kaistia type strain, were 77.3 and 21.1 %, respectively. Major cellular fatty acids of strain LYH11 were cyclo-C19 : 0 ω8c, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), iso-C10 : 0, iso-C17 : 0 3-OH, iso-C17 : 1 ω5c and C18 : 0. Strain LYH11 contained phosphatidylglycerol, phosphatidylethanolamine, an unidentified phosphoaminolipid, an unidentified aminolipid, three unidentified phospholipids and five unidentified lipids as polar lipids. Ubiquinone-10 was the major respiratory quinone. The genomic DNA G+C content was 64.5 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain LYH11 represents a novel species of the genus Kaistia , for which the name Kaistia algarum sp. nov. is proposed. The type strain is LYH11 (=KACC 19096=JCM 31803).

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2018-07-31
2019-12-15
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