1887

Abstract

A novel Saccharopolyspora strain, designated 3SS5-12, isolated from mangrove sediment collected from Ranong Province is described. The strain was characterized by pale yellow branching aerial mycelium which differentiated into flexuous chains of spores covered with tufts of short curved hairs. The whole-cell hydrolysates of the strain contained meso-diaminopimelic acid as the diagnostic diamino acid, with arabinose, galactose and ribose as the main sugars. A major menaquinone of this strain was MK-9(H4). Mycolic acids were absent. The DNA G+C content of the genomic DNA was 69.4 mol%. The predominant cellular fatty acids were iso-C16 : 0 and anteiso-C17 : 0. Polar lipids consisted of diphosphatidylglycerol, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, unidentified phospholipids and unidentified lipids. Phylogenetic determination based on 16S rRNA gene sequences indicated that the organism was classified in the genus Saccharopolyspora and highly similar to Saccharopolyspora jiangxiensis W12 (98.8 % sequence similarity), Saccharopolyspora hirsuta subsp. kobensis JCM 9109 (98.8 %), Saccharopolyspora antimicrobica I05-00074 (98.2 %) and Saccharopolyspora indica VRC122 (98.1 %). Evidence from the chemotaxonomic, phenotypic and molecular systematic data indicated that strain 3SS5-12 should be classified as a representing novel species of the genus Saccharopolyspora , for which the name Saccharopolyspora maritima sp. nov. is proposed. The type strain is 3SS5-12 (=TBRC 7048=NBRC 112863).

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2018-07-30
2019-10-19
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References

  1. Warwick S, Bowen T, McVeigh H, Embley TM. A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 1994;44:293–299 [CrossRef][PubMed]
    [Google Scholar]
  2. Lacey J, Goodfellow M, Lacy J, Goodfellow M. A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et. sp. nov. J Gen Microbiol 1975;88:75–85 [CrossRef][PubMed]
    [Google Scholar]
  3. Kim SB, Goodfellow M. Genus XIV. Saccharopolyspora. In Whitman WB, Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME et al. (editors) Bergey's Manual of Systematic Bacteriology New York: Springer; 2012; pp.1396–1407
    [Google Scholar]
  4. Pimentel-Elardo SM, Tiro LP, Grozdanov L, Hentschel U. Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 2008;58:628–632 [CrossRef][PubMed]
    [Google Scholar]
  5. Duangmal K, Mingma R, Thamchaipenet A, Matsumoto A, Takahashi Y. Saccharopolyspora phatthalungensis sp. nov., isolated from rhizosphere soil of Hevea brasiliensis. Int J Syst Evol Microbiol 2010;60:1904–1908 [CrossRef][PubMed]
    [Google Scholar]
  6. Guan TW, Wu N, Xia ZF, Ruan JS, Zhang XP et al. Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China. Extremophiles 2011;15:373–378 [CrossRef][PubMed]
    [Google Scholar]
  7. Cheng J, Zhang YG, Chen W, Li L, Zhang DF et al. Saccharopolyspora cavernae sp. nov., a novel actinomycete isolated from the Swallow Cave in Yunnan, south-west China. Antonie van Leeuwenhoek 2013;104:837–843 [CrossRef][PubMed]
    [Google Scholar]
  8. Zhang YJ, Zhang WD, Qin S, Bian GK, Xing K et al. Saccharopolyspora dendranthemae sp. nov. a halotolerant endophytic actinomycete isolated from a coastal salt marsh plant in Jiangsu, China. Antonie van Leeuwenhoek 2013;103:1369–1376 [CrossRef][PubMed]
    [Google Scholar]
  9. Meklat A, Bouras N, Zitouni A, Sabaou N, Mathieu F et al. Saccharopolyspora ghardaiensis sp. nov., an extremely halophilic actinomycete isolated from Algerian Saharan soil. J Antibiot 2014;67:299–303 [CrossRef][PubMed]
    [Google Scholar]
  10. Suksaard P, Pathom-Aree W, Duangmal K. Diversity and plant growth promoting activities of actinomycetes from mangroves. Chiang Mai J Sci 2017;44:1210–1223
    [Google Scholar]
  11. Kuester E, Williams ST. Selection of media for isolation of Streptomycetes. Nature 1964;202:928–929 [CrossRef][PubMed]
    [Google Scholar]
  12. Mingma R, Pathom-Aree W, Trakulnaleamsai S, Thamchaipenet A, Duangmal K. Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen, Xanthomonas campestris pv. glycine. World J Microbiol Biotechnol 2014;30:271–280 [CrossRef][PubMed]
    [Google Scholar]
  13. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  14. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  15. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  16. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  18. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolis New York: Academic Press; 1969; pp.21–132
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  20. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  21. Mundie DA. The NBS/ISCC Color System Pittsburgh, PA: PolymathSystems; 535.6 dc–20 1995
    [Google Scholar]
  22. Gordon RE, Mihm JM. A comparative study of some strains received as nocardiae. J Bacteriol 1957;73:15–27[PubMed]
    [Google Scholar]
  23. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974;24:54–63 [CrossRef]
    [Google Scholar]
  24. Becker B, Lechevalier MP, Lechevalier HA. Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 1965;13:236–243[PubMed]
    [Google Scholar]
  25. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983;29:319–322 [CrossRef]
    [Google Scholar]
  26. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  27. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  28. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977;27:104–117 [CrossRef]
    [Google Scholar]
  29. Tomiyasu I. Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J Bacteriol 1982;151:828–837[PubMed]
    [Google Scholar]
  30. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984;25:125–128 [CrossRef]
    [Google Scholar]
  31. Wang C, Xu XX, Qu Z, Wang HL, Lin HP et al. Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 2011;61:320–324 [CrossRef][PubMed]
    [Google Scholar]
  32. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987;37:463–464
    [Google Scholar]
  33. Zhang J, Wu D, Liu Z. Saccharopolyspora jiangxiensis sp. nov., isolated from grass-field soil. Int J Syst Evol Microbiol 2009;59:1076–1081 [CrossRef][PubMed]
    [Google Scholar]
  34. Iwasaki A, Itoh H, Mori T. A new broad-spectrum aminoglycoside antibiotic complex, sporaricin. II. Taxonomic studies on the sporaricin producing strain Saccharopolyspora hirsuta subsp. kobensis nov. subsp. J Antibiot 1979;32:180–186 [CrossRef][PubMed]
    [Google Scholar]
  35. Yuan LJ, Zhang YQ, Guan Y, Wei YZ, Li QP et al. Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 2008;58:1180–1185 [CrossRef][PubMed]
    [Google Scholar]
  36. Vaddavalli R, Peddi S, Kothagauni SY, Begum Z, Gaddam B et al. Saccharopolyspora indica sp. nov., an actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis). Int J Syst Evol Microbiol 2014;64:1559–1565 [CrossRef][PubMed]
    [Google Scholar]
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