Sphaerisporangium rhizosphaerae sp. nov., an actinomycete isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) Free

Abstract

A novel actinomycete, designated strain NEAU-mq3, was isolated from the rhizosphere soil of a rubber tree (Hevea brasiliensis Muell. Arg) collected from Xianglu Mountain in Heilongjiang Province, north-east China, and characterized by using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and that it forms a monophyletic clade with its closest relatives ‘ Sphaerisporangium dianthi ’ NEAU-CY18 (99.2 % 16S rRNA gene sequence similarity) and Sphaerisporangium cinnabarinum JCM 3291 (98.8 %). Morphological and chemotaxonomic properties of strain NEAU-mq3 were also consistent with the description of the genus Sphaerisporangium . The whole-cell sugars were madurose, mannose, ribose and glucose. The menaquinones were MK-9(H2), MK-9(H4), MK-9(H0) and MK-9(H6). The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol mannoside, an unidentified polar lipid and an unidentified phospholipid. The major fatty acids were identified as iso-C16 : 0, 10-methyl C17 : 0, C16 : 1ω7c and C17 : 1ω7c. DNA–DNA hybridization experiments and phenotypic tests were carried out between strain NEAU-mq3 and its most closely related strains, which further clarified their relatedness and demonstrated that NEAU-mq3 could be distinguished from these strains. Therefore, it is concluded that strain NEAU-mq3 represents a novel species of the genus Sphaerisporangium , for which the name Sphaerisporangium rhizosphaerae sp. nov. is proposed. The type strain is NEAU-mq3 (=CGMCC 4.7429=JCM 32389).

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2018-07-16
2024-03-28
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