1887

Abstract

A Gram-positive and non-motile actinobacterium, designated strain EGI 60016, was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Xinyuan County, Xinjiang Province, China. The 16S rRNA gene sequence of strain EGI 60016 was found to show 97.5 and 97.3 % sequence similarities to Nesterenkonia rhizosphaerae EGI 80099 and Nesternkonia massiliensis NP1, respectively. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60016 formed a distinct clade with N. rhizosphaerae EGI 80099 and N. massiliensis NP1. The polar lipids detected for strain EGI 60016 were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, an unidentified lipid and an unidentified phospholipid. The DNA G+C content was determined to be 64.1 mol%. Other chemotaxonomic features of strain EGI 60016 included MK-7, MK-8 and MK-9 as the respiratory quinones, and anteiso-C15 : 0 and anteiso-C17 : 0 as the major fatty acids. Based on the results of the phylogenetic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60016 is considered to represent a novel species of the genus Nesterenkonia , for which the name Nesterenkonia endophytica sp. nov. is proposed. The type strain is EGI 60016 (=CCTCC AB 2017176=NBRC 112398).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002905
2018-07-03
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/8/2659.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002905&mimeType=html&fmt=ahah

References

  1. Stackebrandt E, Koch C, Gvozdiak O, Schumann P. Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 1995; 45:682–692 [View Article][PubMed]
    [Google Scholar]
  2. Collins MD, Lawson PA, Labrenz M, Tindall BJ, Weiss N et al. Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 2002; 52:1145–1150 [View Article][PubMed]
    [Google Scholar]
  3. Li WJ, Chen HH, Kim CJ, Zhang YQ, Park DJ et al. Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 2005; 55:463–466 [View Article][PubMed]
    [Google Scholar]
  4. Finore I, Orlando P, di Donato P, Leone L, Nicolaus B et al. Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica. Int J Syst Evol Microbiol 2016; 66:1554–1560 [View Article][PubMed]
    [Google Scholar]
  5. Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VR. Nesterenkonia cremea sp. nov., a bacterium isolated from a soda lake. Int J Syst Evol Microbiol 2017; 67:1861–1866 [View Article][PubMed]
    [Google Scholar]
  6. Borsodi AK, Szili-Kovács T, Schumann P, Spröer C, Márialigeti K et al. Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium. Int J Syst Evol Microbiol 2017; 67:4116–4120 [View Article][PubMed]
    [Google Scholar]
  7. Liu JM, Tuo L, Habden X, Guo L, Jiang ZK et al. Nesterenkonia populi sp. nov., an actinobacterium isolated from Populus euphratica. Int J Syst Evol Microbiol 2015; 65:1474–1479 [View Article][PubMed]
    [Google Scholar]
  8. Qin S, Li J, Chen HH, Zhao GZ, Zhu WY et al. Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 2009; 75:6176–6186 [View Article][PubMed]
    [Google Scholar]
  9. Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985; 49:1–7[PubMed]
    [Google Scholar]
  10. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1618 [View Article][PubMed]
    [Google Scholar]
  12. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article][PubMed]
    [Google Scholar]
  13. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  16. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  17. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  18. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  19. Ming H, Nie GX, Jiang HC, Yu TT, Zhou EM et al. Paenibacillus frigoriresistens sp. nov., a novel psychrotroph isolated from a peat bog in Heilongjiang, Northern China. Antonie van Leeuwenhoek 2012; 102:297–305 [View Article][PubMed]
    [Google Scholar]
  20. Leifson E. Atlas of Bacterial Flagellation London: Academic Press; 1960
    [Google Scholar]
  21. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  22. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703–704 [View Article][PubMed]
    [Google Scholar]
  23. Smibert R, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  24. Reddy GS, Nagy M, Garcia-Pichel F. Belnapia moabensis gen. nov., sp. nov., an alphaproteobacterium from biological soil crusts in the Colorado Plateau, USA. Int J Syst Evol Microbiol 2006; 56:51–58 [View Article][PubMed]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: Microbial ID, Inc; 1990
    [Google Scholar]
  26. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  27. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980; 48:459–470 [View Article]
    [Google Scholar]
  28. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  29. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  30. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477[PubMed]
    [Google Scholar]
  31. Tang SK, Wang Y, Chen Y, Lou K, Cao LL et al. Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol 2009; 59:2025–2032 [View Article][PubMed]
    [Google Scholar]
  32. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  33. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  34. Edouard S, Sankar S, Dangui NP, Lagier JC, Michelle C et al. Genome sequence and description of Nesterenkonia massiliensis sp. nov. strain NP1T. Stand Genomic Sci 2014; 9:866–882 [View Article][PubMed]
    [Google Scholar]
  35. Wang HF, Zhang YG, Chen JY, Hozzein WN, Li L et al. Nesterenkonia rhizosphaerae sp. nov., an alkaliphilic actinobacterium isolated from rhizosphere soil in a saline-alkaline desert. Int J Syst Evol Microbiol 2014; 64:4021–4026 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002905
Loading
/content/journal/ijsem/10.1099/ijsem.0.002905
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error