1887

Abstract

A novel bacterial strain, S-12, of a member of the genus Phreatobacter was isolated from a cathode of a microbial fuel cell from Suwon City, South Korea. Cells were Gram-staining-negative, aerobic, non-sporulating rods, motile by means of a polar flagellum, and formed white round colonies. The strain grew at the range of 10–40 °C (optimum, 28–30 °C), pH 6.0–10.0 (optimum 7.0–8.0) and 0–1 % NaCl. The 16S rRNA gene sequence analysis showed the relatedness of S-12 to Phreatobacter stygius YC6-17 (98.2 %) and Phreatobacter oligotrophus PI_21 (98.1 %). The major respiratory quinone was ubiquinone Q-10. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified lipid. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The DNA G+C content was 69.3 mol%. On the basis of its differences from species of the genus Phreatobacter with validly published names, strain S-12 is identified as representing a novel species, for which the proposed name is Phreatobacter cathodiphilus sp. nov., with S-12 as the type strain (=KACC 18497=JCM 31612).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002904
2018-07-17
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/9/2855.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002904&mimeType=html&fmt=ahah

References

  1. Tóth EM, Vengring A, Homonnay ZG, Kéki Z, Spröer C et al. Phreatobacter oligotrophus gen. nov., sp. nov., an alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant. Int J Syst Evol Microbiol 2014;64:839–845 [CrossRef][PubMed]
    [Google Scholar]
  2. Lee SD, Joung Y, Cho JC. Phreatobacter stygius sp. nov., isolated from pieces of wood in a lava cave and emended description of the genus Phreatobacter. Int J Syst Evol Microbiol 2017;67:3296–3300 [CrossRef][PubMed]
    [Google Scholar]
  3. Nitisoravut R, Regmi R. Plant microbial fuel cells: a promising biosystems engineering. Renew Sust Energ Rev 2017;76:81–89 [CrossRef]
    [Google Scholar]
  4. Ahn JH, Jeong WS, Choi MY, Kim BY, Song J et al. Phylogenetic diversity of dominant bacterial and archaeal communities in plant–microbial fuel cells using rice plants. J Microbiol Biotechnol 2014;24:1707–1718 [CrossRef][PubMed]
    [Google Scholar]
  5. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 2013;10:563–569 [CrossRef][PubMed]
    [Google Scholar]
  6. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014;30:2068–2069 [CrossRef][PubMed]
    [Google Scholar]
  7. Lagesen K, Hallin P, Rødland EA, Staerfeldt HH, Rognes T et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007;35:3100–3108 [CrossRef][PubMed]
    [Google Scholar]
  8. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  9. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012;28:1823–1829 [CrossRef][PubMed]
    [Google Scholar]
  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  11. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  13. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  14. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68:461–466 [CrossRef][PubMed]
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  16. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002904
Loading
/content/journal/ijsem/10.1099/ijsem.0.002904
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error