1887

Abstract

A Gram-negative, strictly aerobic, oxidase-positive, catalase-negative and yellow-pigmented bacterium, designated strain HR-AV, was isolated from a water sample of the Han River. Cells were elongated rods with gliding motility without flagellum. Growth was observed at 5–30 °C (optimum, 20 °C), pH 7–8 and 0–0.5 % NaCl. The major respiratory quinone was menaquinone-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and anteiso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, an unidentified amino lipid and five unidentified lipids. The DNA G+C content of strain HR-AV was 38.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AVbelonged to the family Sphingobacteriaceae in the phylum Bacteroidetes and formed a phylogenic lineage with Solitalea canadensis DSM 3403 and Solitalea koreensis R2A36-4. Strain HR-AV was most closely related to S. canadensis DSM 3403 and S. koreensis R2A36-4 with 97.3 and 94.0 % 16S rRNA gene sequence similarities, respectively, and then had low similarities (below 90.9 %) with other bacteria with validly published names. Average nucleotide identity and in silico DNA–DNA hybridization values between strain HR-AV and S. canadensis were 74.0 and 19.7 %, respectively. Based on these results, strain HR-AV represents a novel species of the genus Solitalea , for which the name Solitalea longa sp. nov. is proposed. The type strain is HR-AV (=KACC 19411=JCM 32259). An emended description of the genus Solitalea is also proposed.

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2018-07-17
2019-10-14
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