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Volume 68, Issue 9

Solitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea Free

Abstract

A Gram-negative, strictly aerobic, oxidase-positive, catalase-negative and yellow-pigmented bacterium, designated strain HR-AV, was isolated from a water sample of the Han River. Cells were elongated rods with gliding motility without flagellum. Growth was observed at 5–30 °C (optimum, 20 °C), pH 7–8 and 0–0.5 % NaCl. The major respiratory quinone was menaquinone-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and anteiso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, an unidentified amino lipid and five unidentified lipids. The DNA G+C content of strain HR-AV was 38.4 mol%. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-AVbelonged to the family Sphingobacteriaceae in the phylum Bacteroidetes and formed a phylogenic lineage with Solitalea canadensis DSM 3403 and Solitalea koreensis R2A36-4. Strain HR-AV was most closely related to S. canadensis DSM 3403 and S. koreensis R2A36-4 with 97.3 and 94.0 % 16S rRNA gene sequence similarities, respectively, and then had low similarities (below 90.9 %) with other bacteria with validly published names. Average nucleotide identity and in silico DNA–DNA hybridization values between strain HR-AV and S. canadensis were 74.0 and 19.7 %, respectively. Based on these results, strain HR-AV represents a novel species of the genus Solitalea , for which the name Solitalea longa sp. nov. is proposed. The type strain is HR-AV (=KACC 19411=JCM 32259). An emended description of the genus Solitalea is also proposed.

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Keyword(s): emended description , freshwater , new taxa , Solitalea longa and taxonomy
© 2018 IUMS
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2018-07-17
2024-04-18
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References

  1. Weon HY, Kim BY, Lee CM, Hong SB, Jeon YA et al. Solitalea koreensis gen. nov., sp. nov. and the reclassification of [Flexibacter] canadensis as Solitalea canadensis comb. nov. Int J Syst Evol Microbiol 2009; 59:1969–1975 [View Article][PubMed]
     [Google Scholar]
  2. Steyn PL, Segers P, Vancanneyt M, Sandra P, Kersters K et al. Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 1998; 48:165–177 [View Article][PubMed]
     [Google Scholar]
  3. Krieg NR, Ludwig W, Euzéby J, Whitman WB. Phylum XIV. Bacteroidetes phyl. nov. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York: Springer; 2011 p. 25
     [Google Scholar]
  4. Christensen P. Flexibacter canadensis sp. nov. Int J Syst Bacteriol 1980; 30:429–432
     [Google Scholar]
  5. Bae JY, Kim KY, Kim JH, Lee K, Cho JC et al. Paenibacillus aestuarii sp. nov., isolated from an estuarine wetland. Int J Syst Evol Microbiol 2010; 60:644–647 [View Article][PubMed]
     [Google Scholar]
  6. Choi MJ, Bae JY, Kim KY, Kang H, Cha CJ. Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland. Int J Syst Evol Microbiol 2010; 60:1595–1599 [View Article][PubMed]
     [Google Scholar]
  7. Lee JJ, Lee YH, Park SJ, Lee SY, Kim BO et al. Spirosoma knui sp. nov., a radiation-resistant bacterium isolated from the Han River. Int J Syst Evol Microbiol 2017; 67:1359–1365 [View Article][PubMed]
     [Google Scholar]
  8. Lee JJ, Park SJ, Lee YH, Lee SY, Ten LN et al. Hymenobacter aquaticus sp. nov., a radiation-resistant bacterium isolated from a river. Int J Syst Evol Microbiol 2017; 67:1206–1211 [View Article][PubMed]
     [Google Scholar]
  9. Park SJ, Choi JH, Cha CJ. Chryseobacterium rigui sp. nov., isolated from an estuarine wetland. Int J Syst Evol Microbiol 2013; 63:1062–1067 [View Article][PubMed]
     [Google Scholar]
  10. Dorch M, Stackebrandt E. Some modifications in the procedure of direct sequencing of PCR amplified 16s rDNA. J Microbiol Methods 1992; 16:271–279
     [Google Scholar]
  11. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  12. Lee Y, Jeon CO. Sphingomonas frigidaeris sp. nov., isolated from an air conditioning system. Int J Syst Evol Microbiol 2017; 67:3907–3912 [View Article][PubMed]
     [Google Scholar]
  13. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article][PubMed]
     [Google Scholar]
  14. Felsenstein J. PHYLIP (Phylogeny Inference Package), Version 3.6a Seattle: Department of genetics, University of Washington, Seattle, WA, USA; 2002
     [Google Scholar]
  15. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
     [Google Scholar]
  16. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 2007; 73:5261–5267 [View Article][PubMed]
     [Google Scholar]
  17. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
     [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
     [Google Scholar]
  19. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article][PubMed]
     [Google Scholar]
  20. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article][PubMed]
     [Google Scholar]
  21. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1988; 19:1–67
     [Google Scholar]
  22. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  23. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988; 19:161–208
     [Google Scholar]
  24. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  25. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117
     [Google Scholar]
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Solitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea
Int J Syst Evol Microbiol 68, 2826 (2018); https://doi.org/10.1099/ijsem.0.002903
/content/journal/ijsem/10.1099/ijsem.0.002903
/content/journal/ijsem/10.1099/ijsem.0.002903
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