@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002899, author = "Zhang, Weiyan and Yuan, Ye and Su, Dengquan and Ding, Lijian and Yan, Xiaojun and Wu, Min and Epstein, Slava S. and He, Shan", title = "Saccharospirillum mangrovi sp. nov., a bacterium isolated from mangrove sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "9", pages = "2813-2818", doi = "https://doi.org/10.1099/ijsem.0.002899", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002899", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Saccharospirillum mangrovi", keywords = "mangrove sediment", keywords = "taxonomy", abstract = "A Gram-negative, spirilla, non-spore-forming, motile and strictly aerobic bacterium, designated HK-33T, was isolated from a mangrove sediment sample in Haikou city, Hainan Province, China. Strain HK-33T was able to grow at 10–45 °C (optimum 37 °C), 0.5–12.0 % (w/v) NaCl (2.0 %, w/v) and pH 5.5–8.5 (pH 7.0). The major cellular fatty acids were C16 : 0 and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). Ubiquinone-8 was the predominant respiratory quinone, and Q-9 was present in trace amounts. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, an unidentified aminophosphoglycolipid and two unidentified lipids. The DNA G+C content was 57.3 mol%. According to 16S rRNA gene sequences similarity, strain HK-33T shared 98.6 %, 96.4%, 95.7 and 94.9 % sequence similarities to the species Saccharospirillum correiae CPA1T, Saccharospirillum impatiens EL-105T, Saccharospirillum salsuginis YIM-Y25T and Saccharospirillum aestuarii IMCC 4453T, respectively. Phylogenetic analysis showed that strain HK-33T was clustered with S. correiae CPA1T, S. impatiens EL-105T, S. salsuginis YIM-Y25T and S. aestuarii IMCC 4453T. Results of DNA–DNA hybridization analysis revealed that strain HK-33T shared 36.3±1.7 % DNA relatedness with S. correiae CPA1T. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain HK-33T is considered to represent a novel species in the genus Saccharospirillum , for which the name Saccharospirillum mangrovi sp. nov. is proposed. The type strain is HK-33T (=KCTC 62178T=MCCC 1K03440T).", }