1887

Abstract

An electrogenic bacterium was isolated from a marine coral, designated as strain JC435 and its taxonomic status examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate belonged to the genus Rhodococcus and formed a cluster with Rhodococcus ruber KCTC 9806 (99.5 % 16S rRNA gene sequence similarity) and Rhodococcus aetherivorans JCM 14343 (99.3 %), respectively. Genome relatedness based on DNA–DNA hybridization to the type strains of closest-related species was less than 30 % and the ΔTm of >7 °C, suggesting that strain represents a new species of the genus Rhodococcus . The major fatty acids were C16 : 0, C18 : 1ω9c, C18 : 010-methyl and C16 : 1ω6c and/or C16 : 1ω7c. The polar lipids of strain JC435 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, phosphatidylinositol, three unknown phospholipids and an unknown amino lipid. The major isoprenoid quinone was MK-8(H2), with 8 % of MK-7(H2) and 2 % of MK-9(H2) as minor components. Whole-cell hydrolysates contained meso-diaminopimelic acid, arabinose and galactose as the diagnostic diamino acid and sugars. Mycolic acids were detected. The genomic DNA G+C content of strain JC435 was 69.8 mol%. On the basis of phylogenetic genotypic, physiological and chemotaxonomic analysis, strain JC435 is considered to represent a novel species of the genus Rhodococcus for which the name Rhodococcus electrodiphilus sp. nov. is proposed. The type strain is JC435 (=KCTC 39856=LMG 29881=MCC 3659).

Keyword(s): electrogenic , Rhodococcus and sp. nov.
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002895
2018-06-29
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/8/2644.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002895&mimeType=html&fmt=ahah

References

  1. Logan BE, Regan JM. Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol 2006;14:512–518 [CrossRef]
    [Google Scholar]
  2. Lovley DR. Bug juice: harvesting electricity with microorganisms. Nat Rev Microbiol 2006;4:497–508 [CrossRef][PubMed]
    [Google Scholar]
  3. Mahidhara G, Chintalapati VR. Eco-physiological and interdisciplinary approaches for empowering biobatteries. Ann Microbiol 2016;66:543–557 [CrossRef]
    [Google Scholar]
  4. Thauer RK, Jungermann K, Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev 1977;41:100–180
    [Google Scholar]
  5. Zopf W. Uber Ausscheidung von Fettfarbstoffen (Lipochromen) seitens gewisser Spaltpilze. Ber Deut Bot Ges 1891;9:22–28
    [Google Scholar]
  6. Hwang CY, Lee I, Cho Y, Lee YM, Baek K et al. Rhodococcus aerolatus sp. nov., isolated from subarctic rainwater. Int J Syst Evol Microbiol 2015;65:465–471 [CrossRef][PubMed]
    [Google Scholar]
  7. Kampfer P, Dott W, Martin K, Glaeser SP. Rhodococcus defluvii sp. nov., isolated from wastewater of a bioreactor and formal proposal to reclassify [Corynebacterium hoagii] and Rhodococcus equi as Rhodococcus hoagii comb. nov. Int J Syst Evol Microbiol 2014;64:755–761 [CrossRef]
    [Google Scholar]
  8. Jones AL, Goodfellow M. Genus IV. Rhodococcus (Zopf 1891) emend. Goodfellow, Alderson and Chun 1998a. In Goodfellow M, Kampfer P, Busse H-J, Trujillo ME, Suzuki K et al. (editors) Bergey’s Manual of Systematic Bacteriology the Actinobacteria Part A, 2nd ed.vol. 5 London, New York: Springer; 2012; pp.437–464
    [Google Scholar]
  9. Goodfellow M, Jones AL, Maldonado LA, Salanitro J. Rhodococcus aetherivorans sp. nov., a new species that contains methyl t-butyl ether-degrading actinomycetes. Syst Appl Microbiol 2004;27:61–65 [CrossRef][PubMed]
    [Google Scholar]
  10. Temmerman W, Vereecke D, Dreesen R, van Montagu M, Holsters M et al. Leafy gall formation is controlled by fasR, an AraC-type regulatory gene in Rhodococcus fascians. J Bacteriol 2000;182:5832–5840 [CrossRef][PubMed]
    [Google Scholar]
  11. Ramaprasad EVV, Bharti D, Sasikala Ch, Ramana Ch V. Zooshikella marina sp. nov. a cycloprodigiosin- and prodigiosin-producing marine bacterium isolated from beach sand. Int J Syst Evol Microbiol 2015;65:4669–4673 [CrossRef][PubMed]
    [Google Scholar]
  12. Ramaprasad EVV, Sasikala Ch, Ramana Ch V. Ornithinimicrobium algicola sp. nov., a marine actinobacterium isolated from the green alga of the genus Ulva. Int J Syst Evol Microbiol 2015;65:4627–4631 [CrossRef][PubMed]
    [Google Scholar]
  13. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  14. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  15. Ramaprasad EVV, Sasikala Ch, Ramana Ch V. Roseomonas oryzae sp. nov., isolated from paddy rhizosphere soil. Int J Syst Evol Microbiol 2015;65:3535–3540 [CrossRef][PubMed]
    [Google Scholar]
  16. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S et al. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  19. Ramaprasad EVV, Tushar L, Dave B, Sasikala Ch, Ramana Ch V. Rhodovulum algae sp. nov., isolated from an algal mat. Int J Syst Evol Microbiol 2016;66:3367–3371 [CrossRef][PubMed]
    [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  21. Kates M. Techniques of lipidology. In Burdon H, van Knippenberg PH. (editors) Laboratory Techniques in Biochemistry and Molecular Biology Amsterdam, The Netherlands: Elsevier; 1986; pp.100–110
    [Google Scholar]
  22. Mahidhara G, Sasikala Ch, Ramana Ch V. Comparative metabolomic studies of Alkanivorax xenomutans showing differential power output in a three chambered microbial fuel cell. World J Microbiol Biotechnol 2017;33:102 [CrossRef][PubMed]
    [Google Scholar]
  23. Stackebrandt E, Goebel BM. Taxonomic Note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994;44:846–849 [CrossRef]
    [Google Scholar]
  24. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
  25. Rosselló-Mora R, Amann R. The species concept for prokaryotes. FEMS Microbiol Rev 2001;25:39–67 [CrossRef][PubMed]
    [Google Scholar]
  26. Subhash Y, Sasikala Ch, Ramana Ch V. Sphingopyxis contaminans sp. nov., isolated from a contaminated Petri dish. Int J Syst Evol Microbiol 2014;64:2238–2243 [CrossRef][PubMed]
    [Google Scholar]
  27. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980;188:221–233 [CrossRef]
    [Google Scholar]
  28. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970;20:435–443 [CrossRef]
    [Google Scholar]
  29. Mahidhara G, Chintalapati VR. Eco-physiological and interdisciplinary approaches for empowering biobatteries. Ann Microbiol 2015;66:543–557 [CrossRef]
    [Google Scholar]
  30. Wang H, Correa E, Dunn WB, Winder CL, Goodacre R et al. Metabolomic analyses show that electron donor and acceptor ratios control anaerobic electron transfer pathways in Shewanella oneidensis. Metabolomics 2013;9:642–656 [CrossRef]
    [Google Scholar]
  31. Zhang J, Burgess JG. Shewanella electrodiphila sp. nov., a psychrotolerant bacterium isolated from Mid-Atlantic Ridge deep-sea sediments. Int J Syst Evol Microbiol 2015;65:2882–2889 [CrossRef]
    [Google Scholar]
  32. Sydow A, Krieg T, Mayer F, Schrader J, Holtmann D et al. Electroactive bacteria–molecular mechanisms and genetic tools. Appl Microbiol Biotechnol 2014;98:8481–8495 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002895
Loading
/content/journal/ijsem/10.1099/ijsem.0.002895
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error