%0 Journal Article %A Dong, Yingying %A Liu, Yuhui %A Chen, Nanchun %A Zhong, Yijian %A Liu, Li %A Xie, Qinglin %T Clostridium beihaiense sp. nov., an anaerobic bacterium isolated from activated sludge %D 2018 %J International Journal of Systematic and Evolutionary Microbiology, %V 68 %N 9 %P 2789-2793 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.002885 %K anaerobe %K new species %K taxonomy %K Clostridium beihaiense sp. nov. %I Microbiology Society, %X A Gram-positive, strictly anaerobic, rod-shaped bacterium, designated YB-7T, was isolated from activated sludge of an anaerobic baffled reactor pond in Weizhou terminal wastewater treatment plant, Beihai, Guangxi, China. Strain YB-7T grew at pH 5.0–12.0 (optimum, pH 7.0), 20–45 °C (37 °C) and NaCl concentration of 0–5 % w/v (optimum, 5 %). 16S rRNA gene sequence analysis results showed that strain YB-7T belonged to the genus Clostridium and it was most closely related to Clostridium tetanomorphum DSM 4474T (96.9 % similarity). The DNA–DNA relatedness of strain YB-7T to Clostridium tetanomorphum DSM 4474T was 47.4 %. The DNA G+C content of strain YB-7T was determined to be 32.3 mol%, and the predominant cellar fatty acid (>10 %) was C16 : 0. Polar lipids of strain YB-7T included diphosphatidylglycerol, phosphatidylethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids, two unidentified phospholipids and unidentified lipids. The results of this study supported the conclusion that strain YB-7T should be assigned to a new member of the genus Clostridium , for which the name Clostridium beihaiense sp. nov. is proposed. The type strain is YB-7T (=CICC 24109T=KCTC 15555T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002885