1887

Abstract

A Gram-positive, strictly anaerobic, rod-shaped bacterium, designated YB-7, was isolated from activated sludge of an anaerobic baffled reactor pond in Weizhou terminal wastewater treatment plant, Beihai, Guangxi, China. Strain YB-7 grew at pH 5.0–12.0 (optimum, pH 7.0), 20–45 °C (37 °C) and NaCl concentration of 0–5 % w/v (optimum, 5 %). 16S rRNA gene sequence analysis results showed that strain YB-7 belonged to the genus Clostridium and it was most closely related to Clostridium tetanomorphum DSM 4474 (96.9 % similarity). The DNA–DNA relatedness of strain YB-7 to Clostridium tetanomorphum DSM 4474 was 47.4 %. The DNA G+C content of strain YB-7 was determined to be 32.3 mol%, and the predominant cellar fatty acid (>10 %) was C16 : 0. Polar lipids of strain YB-7 included diphosphatidylglycerol, phosphatidylethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminophospholipids, two unidentified phospholipids and unidentified lipids. The results of this study supported the conclusion that strain YB-7 should be assigned to a new member of the genus Clostridium , for which the name Clostridium beihaiense sp. nov. is proposed. The type strain is YB-7 (=CICC 24109=KCTC 15555).

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2018-07-20
2024-12-09
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References

  1. Yu L, Han M, He F. A review of treating oily wastewater. Arab J Chem 2017; 102:1913–1922
    [Google Scholar]
  2. Zhang H, Chen J, Liu YM, Xie QL, You SH et al. Engineering practice of treating oil-field wastewater with high salinity and chlorine. Chin Water Wastewater 2006; 22:71–73
    [Google Scholar]
  3. Tan ZH, Liu L, Liu YH, Zhang CF, Wang S et al. Performance and microbial community analysis of oil-field wastewater treatment system in Weizhou. Environ Eng 2016; 34:6–10
    [Google Scholar]
  4. Liu Y, Liu L, Wang S, Zhang C, Tan Z et al. Characteristics of microbial community in anaerobic ABR system for treating oil-field wastewater. Ind Saf Environ Prot 2016; 42:63–67
    [Google Scholar]
  5. Lawson P. The taxonomy of the genus Clostridium: current status and future perspectives. Microbiol Chin 2016; 43:1070–1074
    [Google Scholar]
  6. Lawson PA, Rainey FA. Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. Int J Syst Evol Microbiol 2016; 66:1009–1016 [View Article][PubMed]
    [Google Scholar]
  7. Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J et al. The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 1994; 44:812–826 [View Article][PubMed]
    [Google Scholar]
  8. Genus Clostridium 2018 www.bacterio.net/clostridium.html
  9. Parte AC. LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
    [Google Scholar]
  10. George WL, Sutter VL, Citron D, Finegold SM. Selective and differential medium for isolation of Clostridium difficile. J Clin Microbiol 1979; 9:214–219[PubMed]
    [Google Scholar]
  11. Kim S, Jeong H, Chun J. Clostridium aestuarii sp. nov., from tidal flat sediment. Int J Syst Evol Microbiol 2007; 57:1315–1317 [View Article][PubMed]
    [Google Scholar]
  12. Moyes RB, Reynolds J, Breakwell DP. Differential Staining of Bacteria: Gram Stain vol. appendix 3 Wiley; 2009 pp. A.3C.1-8
    [Google Scholar]
  13. Wang Q, Wang CD, Li CH, Li JG, Chen Q et al. Clostridium luticellarii sp. nov., isolated from a mud cellar used for producing strong aromatic liquors. Int J Syst Evol Microbiol 2015; 65:4730–4733 [View Article][PubMed]
    [Google Scholar]
  14. Krist KA, Ross T, McMeekin TA. Final optical density and growth rate; effects of temperature and NaCl differ from acidity. Int J Food Microbiol 1998; 43:195–203 [View Article][PubMed]
    [Google Scholar]
  15. Gresser ME, Shanholtzer CJ, Gerding DN, Garrett CR, Peterson LR. Evaluation of the 24-h API 20A anaerobe system for identification of Clostridium difficile. J Clin Microbiol 1984; 19:915–916[PubMed]
    [Google Scholar]
  16. Humble MW, King A, Phillips I. API ZYM: a simple rapid system for the detection of bacterial enzymes. J Clin Pathol 1977; 30:275–277 [View Article][PubMed]
    [Google Scholar]
  17. Ling WH, Saxelin M, Hanninen O, Salminen S. Enzyme profile of Lactobacillus strain GG by a rapid API ZYM system: a comparison of intestinal bacterials strains. Microb Ecol Health D 2009; 7:99–104
    [Google Scholar]
  18. Ausubel FM. Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology vol. 1(4) Wiley; 2002 p. 286
    [Google Scholar]
  19. Akasaka H, Izawa T, Ueki K, Ueki A. Phylogeny of numerically abundant culturable anaerobic bacteria associated with degradation of rice plant residue in Japanese paddy field soil. FEMS Microbiol Ecol 2003; 43:149–161 [View Article][PubMed]
    [Google Scholar]
  20. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
    [Google Scholar]
  21. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  22. Rosselló-Mora R. DNA-DNA reassociation methods applied to microbial taxonomy and their critical evaluation. Springer 2006
    [Google Scholar]
  23. Jahnke K-D. BASIC computer program for evaluation of spectroscopic DNA renaturation data from Gilford system 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 1992; 15:61–73 [View Article]
    [Google Scholar]
  24. Mesbah M, Whitman WB. Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 1989; 479:297–306 [View Article][PubMed]
    [Google Scholar]
  25. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note. 2001 pp. 101–106
    [Google Scholar]
  26. Rouser G, Fkeischer S, Yamamoto A. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 1970; 5:494–496 [View Article][PubMed]
    [Google Scholar]
  27. Stackebrandt E. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 6:152–155
    [Google Scholar]
  28. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  29. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [View Article][PubMed]
    [Google Scholar]
  30. Nair GB. International committee on systematic bacteriology: subcommittee on the taxonomy of Vibrionaceae. Int J Syst Evol Microbiol 2002; 52:2331–2333 [View Article]
    [Google Scholar]
  31. Cirne DG, Delgado OD, Marichamy S, Mattiasson B. Clostridium lundense sp. nov., a novel anaerobic lipolytic bacterium isolated from bovine rumen. Int J Syst Evol Microbiol 2006; 56:625–628 [View Article][PubMed]
    [Google Scholar]
  32. Yin Q, Tao Y, Zhu X, Zhou Y, He X et al. Clostridium liquoris sp. nov., isolated from a fermentation pit used for the production of Chinese strong-flavoured liquor. Int J Syst Evol Microbiol 2016; 66:749–754 [View Article][PubMed]
    [Google Scholar]
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