RT Journal Article SR Electronic(1) A1 Cao, Chengliang A1 Sun, Yong A1 Wu, Bo A1 Zhao, Shuai A1 Yuan, Bo A1 Qin, Sheng A1 Jiang, Jihong A1 Huang, YingYR 2018 T1 Actinophytocola glycyrrhizae sp. nov. isolated from the rhizosphere of Glycyrrhiza inflata JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 8 SP 2504 OP 2508 DO https://doi.org/10.1099/ijsem.0.002864 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, aerobic actinomycete, designated strain BMP B8152T, was isolated from the rhizosphere of Glycyrrhiza inflata collected ashore, in Kashi, Xinjiang province, northwest PR China. A polyphasic approach was used to establish the taxonomic position of this strain. BMP B8152T was observed to form non-fragmented substrate mycelium, and relatively scanty aerial mycelium with rod-shaped spores. Cell-wall hydrolysates contained meso-diaminopimelic acid, galactose, arabinose, glucose and rhamnose (trace). Mycolic acids were not detected. The diagnostic phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, ninhydrin-positive phosphoglycolipid and phosphatidylinositol. The predominant menaquinone and fatty acid were MK-9(H4) and iso-branched hexadecanoate (iso-C16 : 0), respectively. The phylogenetic analyses based on the 16S rRNA gene sequences indicated that BMP B8152T formed a distinct monophyletic clade clustered with Actinophytocola timorensis ID05-A0653T (98.8 % 16S rRNA gene sequence similarity), Actinophytocola oryzae GMKU 367T (98.6 %), Actinophytocola corallina ID06-A0464T (98.2 %) and Actinophytocola burenkhanensis MN08-A0203T (97.5 %). In addition, DNA–DNA hybridization values between BMP B8152T and A. timorensis ID05-A0653T(44.2±3.6 %) and A. oryzae GMKU 367T(36.7±2.3 %) were well below the 70 % limit for species identification. The combined phenotypic and genotypic data indicate that the isolate represents a novel species of the genus Actinophytocola , for which the name Actinophytocola glycyrrhizae sp. nov., is proposed, with the type strain BMP B8152T (=KCTC 49002T=CGMCC 4.7433T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002864