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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 8

Nocardioides allogilvus sp. nov., a novel actinobacterium isolated from a karst cave Free

Abstract

A novel actinobacterium, designated strain CFH 30205, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, China. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive, non-motile and rod-shaped. The strain was found to be catalase- and oxidase-positive. Strain CFH 30205 grew optimally at 28 °C, pH 9.0 and in the presence of up to 1.5 % NaCl (w/v). On the basis of 16S rRNA gene sequence analysis, strain CFH 30205 was most closely related to the type strains of Nocardioides terrigena DS-17 (97.6 % sequence similarity) and Nocardioides sediminis MSL-01 (97.0 %). The DNA G+C content was determined to be 69.9 mol%. ll-2,6-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were mannose, xylose and galactose. The major isoprenoid quinone was MK-8 (H4), and the major fatty acids (>10 %) were iso-C16 : 0 and anteiso-C14 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH 30205 merits representation of a novel species of the genus Nocardioides , for which the name Nocardioides allogilvus sp. nov. is proposed. The type strain is CFH 30205 (=KCTC 49020=CGMCC 4.7457).

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Keyword(s): karst cave , Nocardioides allogilvus sp. nov. and polyphasic taxonomy
© 2018 IUMS
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2018-06-21
2024-04-19
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Nocardioides allogilvus sp. nov., a novel actinobacterium isolated from a karst cave
Int J Syst Evol Microbiol 68, 2485 (2018); https://doi.org/10.1099/ijsem.0.002863
/content/journal/ijsem/10.1099/ijsem.0.002863
/content/journal/ijsem/10.1099/ijsem.0.002863
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