1887

Abstract

A bacterial strain, designated D13, was isolated from sandy soil near a stream in Sinan-gun, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-motile and flexible rod-shaped. Growth occurred at 15–35 °C (optimum 30 °C) and pH 6.5–8.0 (pH 7.0). NaCl was not obligatory for growth but could be tolerated at up to 0.5 % (w/v) NaCl. The DNA G+C content of the genomic DNA of strain D13 was 57.7 mol% and a phylogenetic analysis of the 16S rRNA gene sequence revealed that strain D13 formed a distinct evolutionary lineage within the family Rhodanobacteraceae of the order Lysobacterales . Strain D13 showed highest 16S rRNA sequence similarity to Lysobacter hankyongensis KTCe-2 (92.7 %), followed by Luteimonas cucumeris Y4 (92.7 %), Dyella japonica XD53 (92.6 %) and Aquimonas voraii GPTSA 20 (92.5 %). The major cellular fatty acids (>10 % of the total) were iso-C16 : 0, iso-C15 : 0 and summed feature 9 (iso-C17 : 1 ω9с and/or C16 : 0 10-methyl). The respiratory quinone was ubiquinone-8 and the major polar lipids of the isolate consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine. Based on polyphasic analysis, strain D13 could be differentiated from other genera in the family Rhodanobacteraceae , which suggests that strain D13 represents a novel species of a new genus in the family Rhodanobacteraceae , for which the name Ahniella affigens gen. nov., sp. nov. is proposed. The type strain is D13 (=KACC 19270=JCM 31634).

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2018-06-20
2020-01-29
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