1887

Abstract

A novel actinomycete, strain TRM 41368, was isolated from a silt sample from Xiaoerkule lake in Xinjiang province, China, and was examined using a polyphasic approach. Strain TRM 41368 was aerobic, Gram-stain-positive, with an optimum NaCl concentration for growth of 5 % (w/v), and an optimum temperature for growth of 35–37 °C. On the basis of 16S rRNA gene sequence analysis, strain TRM 41368 was most closely related to Glycomycesfuscus TRM 49117 (98.46 % similarity). However, it had a relatively low DNA–DNA relatedness value with G. fuscus TRM 49117 (ANI=70.59 %). The organism had chemical and morphological features typical of the genus Glycomyces . The cell wall of TRM 41368 contained meso-diaminopimelic acid; xylose, ribose and glucose were the major whole-cell sugars. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositolmannosides. The predominant menaquinone was MK-9(H6). The major fatty acids were C18 : 1ω9c, C16 : 0, iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. The G+C content of the DNA was 69.9 mol%. On the basis of the polyphasic evidence, strain TRM 41368 should be designated as a novel species of the genus Glycomyces , for which the name Glycomyces xiaoerkulensis sp. nov. is proposed. The type strain is TRM 41368 (=CCTCC AA 2017005=KCTC 39932).

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2018-07-09
2021-10-19
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