@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002840, author = "Li, Xiaoguang and Wang, Zhikuan and Lu, Fengjuan and Zhang, Heming and Tian, Jiewei and He, Linling and Chu, Yiwen and Tian, Yongqiang", title = "Actinocorallia populi sp. nov., an endophytic actinomycete isolated from a root of Populus adenopoda (Maxim.)", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "7", pages = "2325-2330", doi = "https://doi.org/10.1099/ijsem.0.002840", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002840", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Actinocorallia populi sp. nov", keywords = "endophytic actinobacterium", keywords = "polyphasic taxonomy", abstract = "An endophytic actinobacterium, strain A251T, was isolated from the root of Populus adenopoda Maxim and subjected to characterization using polyphasic taxonomy. Analysis of the 16S rRNA gene sequence revealed that the isolate represented a member of the phylogenetic cluster of the genus Actinocorallia and was most closely related to Actinocorallia aurantiaca JCM 8201T (98.0 %) and Actinocorallia libanotica IFO 10495T (98.0 %). DNA–DNA hybridization values between A251T and these strains were 41.2 % and 45.0 %, respectively. The G+C content of the DNA was 71.5 mol%. Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω9c. The peptidoglycan diamino acid of A251T was meso-diaminopimelic acid and the whole-cell hydrolysates contained glucose and ribose. The major menaquinones were MK-9(H4) and MK-9(H6). The phospholipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an undefined aminophospholipid and two undefined phospholipids. DNA–DNA hybridization data in combination with differences in the biochemical and physiological properties, indicated that A251T should be classified as representing a novel species within the genus Actinocorallia , for which the name Actinocorallia populi sp. nov. is proposed, with A251T (=CGMCC 4.7421T=JCM 32178T) as the type strain.", }