RT Journal Article SR Electronic(1) A1 Amoozegar, Mohammad Ali A1 Shahinpei, Azadeh A1 Makzum, Somaye A1 Rafieyan, Shokufeh A1 Moshtaghi Nikou, Mahdi A1 Spröer, Cathrin A1 Ventosa, AntonioYR 2018 T1 Salipaludibacillus halalkaliphilus sp. nov., a moderately haloalkaliphilic bacterium from a coastal-marine wetland JF International Journal of Systematic and Evolutionary Microbiology, VO 68 IS 7 SP 2214 OP 2219 DO https://doi.org/10.1099/ijsem.0.002814 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, endospore-forming rod-shaped non-motile, moderately halophilic and alkaliphilic bacterium, strain GASy1T, was isolated from a water sample from Gomishan, a marine wetland in Iran. GASy1T required at least 0.5 % (w/v) NaCl for growth and was able to grow at NaCl concentrations of up to 15 % (w/v), with optimum growth occurring at 5 % (w/v) NaCl. The optimum pH and temperature for growth were pH 8.5–9.0 and 30 °C, respectively, while it was able to grow over a pH range and a temperature range of 7.5–10.0 and 4–40 °C, respectively. GASy1T was catalase-positive and oxidase-negative. Analysis of 16S rRNA gene sequences revealed that GASy1T represents a member of the genus Salipaludibacillus , family Bacillaceae within the order Bacillales , showing 97.4 % sequence similarity to Salipaludibacillus neizhouensis JSM 071004T, and 96.2 and 95.7 % sequence similarity to Salipaludibacillus agaradhaerens AC 13T and Salipaludibacillus aurantiacus S9T, respectively. The DNA G+C content of GASy1T was 38.8 mol%. The polar lipids of the strain were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids and its major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C15 : 0. The isoprenoid quinone was MK-7. DNA–DNA hybridization experiments revealed a low level of relatedness between GASy1T and Salipaludibacillus neizhouensis IBRC-M 10892T (18 %). On the basis of a combination of phenotypic, chemotaxonomic and phylogenetic features, GASy1T represents a novel species of the genus Salipaludibacillus , for which the name Salipaludibacillus halalkaliphilus sp. nov. is proposed. The type strain of Salipaludibacillus halalkaliphilus is GASy1T (=IBRC M 10902T=LMG 28385T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002814