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Abstract

A Gram-stain-positive, endospore-forming rod-shaped non-motile, moderately halophilic and alkaliphilic bacterium, strain GASy1, was isolated from a water sample from Gomishan, a marine wetland in Iran. GASy1 required at least 0.5 % (w/v) NaCl for growth and was able to grow at NaCl concentrations of up to 15 % (w/v), with optimum growth occurring at 5 % (w/v) NaCl. The optimum pH and temperature for growth were pH 8.5–9.0 and 30 °C, respectively, while it was able to grow over a pH range and a temperature range of 7.5–10.0 and 4–40 °C, respectively. GASy1 was catalase-positive and oxidase-negative. Analysis of 16S rRNA gene sequences revealed that GASy1 represents a member of the genus , family within the order , showing 97.4 % sequence similarity to JSM 071004, and 96.2 and 95.7 % sequence similarity to AC 13 and S9, respectively. The DNA G+C content of GASy1 was 38.8 mol%. The polar lipids of the strain were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids and its major cellular fatty acids were anteiso-C, C and iso-C. The isoprenoid quinone was MK-7. DNA–DNA hybridization experiments revealed a low level of relatedness between GASy1 and IBRC-M 10892 (18 %). On the basis of a combination of phenotypic, chemotaxonomic and phylogenetic features, GASy1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is GASy1 (=IBRC M 10902=LMG 28385).

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2018-07-01
2020-04-08
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