1887

Abstract

Two bacterial strains were individually isolated from respiratory tracts; the animals were from the Tibet–Qinghai Plateau, PR China. The isolates were Gram-stain-positive, catalase-negative, coccus-shaped, chain-forming organisms. Analysis of 16S rRNA gene sequences indicated that the type strain HTS25 shared 98.0, 97.4, 97.2 and 97.1 % similarity with , , and respectively. Sequence analysis of the and genes indicated that HTS25 was closely related to (similarities of 94.7 and 91.4 % respectively). Analysis of sequences showed interspecies similarity of 84.8 % between HTS25 and . A whole-genome phylogenetic tree reconstructed from 81 core genes from the genomes of 17 members of the genus was used to validate that HTS25 forms a distinct subline from other recognized species of the genus . DNA–DNA hybridization of HTS25 showed a maximum estimated DNA reassociation value of 32.1 % to CCUG 65085. On the basis of the results of phenotypic and phylogenetic analyses, we propose that the two isolates be classified as representing a novel species of the genus , named sp. nov. The type strain is HTS25 (=DSM 101998=CGMCC 1.15531). The genome of sp. nov. strain HTS25 (2 067 971 bp) contains 2001 genes with an average DNA G+C content of 42.7 mol%.

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2018-06-01
2020-01-26
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