1887

Abstract

A novel actinobacterium, designated TRM46399, was isolated from ditch sediment in Xinjiang Province, Northwest China. Phylogenetic, physiological, chemotaxonomic and morphological analyses demonstrated that strain TRM46399 belonged to the genus Streptomyces . The strain was aerobic, Gram-stain-positive, had aerial mycelia that branched monopodially and formed chains of arthrospores. The spores were oval to cylindrical with smooth surfaces. The strain had an optimum NaCl concentration for growth of 5 % (w/v). The whole-cell sugar pattern of TRM46399 consisted of ribose, xylose, mannose and galactose. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H10). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Major fatty acids were iso-C16 : 0 (47.8 %), iso-C14 : 0 (13.5 %), C16 : 0 (8.9 %), anteiso-C15 : 0 (8.4 %), anteiso-C17 : 0 (4.2 %) and C17 : 0cyclo (2.7 %). The G+C content of the genomic DNA was 68.7 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain TRM46399 (KY688182) was phylogenetically most closely related to Streptomyces xinghaiensis S187 (99.13 % sequence similarity); however, DNA–DNA hybridization studies between stain TRM46399 and S. xinhaiensis S187 showed only 65.32 % relatedness. Based on the evidence from this polyphasic study, strain TRM46399 represents a novel species of the genus Streptomyces , for which the name Streptomyces aqsuensis sp. nov. is proposed. The type strain is TRM46399 (=KCTC 39610 =CCTCC AA 2015009).

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2018-05-31
2019-10-15
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