1887

Abstract

A Gram-stain-negative, aerobic, ovoid or short rod-shaped bacterium with prosthecates and flagella, designated SY-3-19, was isolated from the surface seawater of the South China Sea, and subjected to a polyphasic taxonomic study. The isolate grew at 4–40 °C and pH 5.0–9.0 (optimum 28 °C and pH 6.5–7.5), and with 0.5–16.0 % (w/v) NaCl (optimum 4 %). It was positive for oxidase and catalase activity. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SY-3-19 constituted a separate branch in the family , sharing the highest sequence similarities to the genera (91.9 %), (91.1 %) and (91.0–89.4 %). The sole respiratory quinone was ubiquinone-10 and the principal fatty acids (>10 %) were summed feature 8 (Cω7 and/or Cω6), summed feature 3 (C 7 and/or C 6), 11 methyl C 7 and C. The polar lipids of strain SY-3-19 consisted of phosphatidylglycerol, nine unidentified glycolipids and four unidentified lipids. The DNA G+C content was 60.9 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain SY-3-19 is described as a novel species in a novel genus, for which the name gen. nov., sp. nov. (type strain SY-3-19=MCCC 1K03432=KCTC 62156) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002795
2018-06-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/6/2061.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002795&mimeType=html&fmt=ahah

References

  1. Cho JC, Giovannoni SJ. Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the alpha-Proteobacteria. Int J Syst Evol Microbiol 2003;53:1031–1036 [CrossRef][PubMed]
    [Google Scholar]
  2. Zhen-Li Z, Xin-Qi Z, Nan W, Wen-Wu Z, Xu-Fen Z et al. Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 2014;64:2805–2811 [CrossRef][PubMed]
    [Google Scholar]
  3. Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC et al. Aquisalinus flavus gen. nov., sp. nov., a member of the family Parvularculaceae isolated from a saline lake. Int J Syst Evol Microbiol 2016;66:1813–1817 [CrossRef][PubMed]
    [Google Scholar]
  4. Arun AB, Chen WM, Lai WA, Chou JH, Rekha PD et al. Parvularcula lutaonensis sp. nov., a moderately thermotolerant marine bacterium isolated from a coastal hot spring. Int J Syst Evol Microbiol 2009;59:998–1001 [CrossRef][PubMed]
    [Google Scholar]
  5. Yu Z, Lai Q, Li G, Shao Z. Parvularcula dongshanensis sp. nov., isolated from soft coral. Int J Syst Evol Microbiol 2013;63:2114–2117 [CrossRef][PubMed]
    [Google Scholar]
  6. Zhang XQ, Wu YH, Zhou X, Zhang X, Xu XW et al. Parvularcula flava sp. nov., an alphaproteobacterium isolated from surface seawater of the South China Sea. Int J Syst Evol Microbiol 2016;66:3498–3502 [CrossRef][PubMed]
    [Google Scholar]
  7. Pan J, Sun C, Zhang XQ, Huo YY, Zhu XF et al. Paracoccus sediminis sp. nov., isolated from Pacific Ocean marine sediment. Int J Syst Evol Microbiol 2014;64:2512–2516 [CrossRef][PubMed]
    [Google Scholar]
  8. Yu XY, Tao TY, Fu GY, Su Y, Han SB et al. Marinibaculum pumilum gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2016;66:4844–4849 [CrossRef][PubMed]
    [Google Scholar]
  9. Nokhal T-H, Schlegel HG. Taxonomic study of Paracoccus denitrificans. Int J Syst Bacteriol 1983;33:26–37 [CrossRef]
    [Google Scholar]
  10. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press; 2001
    [Google Scholar]
  11. Zhang WY, Huo YY, Zhang XQ, Zhu XF, Wu M. Halolamina salifodinae sp. nov. and Halolamina salina sp. nov., two extremely halophilic archaea isolated from a salt mine. Int J Syst Evol Microbiol 2013;63:4380–4385 [CrossRef][PubMed]
    [Google Scholar]
  12. Park SC, Baik KS, Kim MS, Kim SS, Kim SR et al. Aequorivita capsosiphonis sp. nov., isolated from the green alga Capsosiphon fulvescens, and emended description of the genus Aequorivita. Int J Syst Evol Microbiol 2009;59:724–728 [CrossRef][PubMed]
    [Google Scholar]
  13. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Meth Microbiol 1987;19:161–207[Crossref]
    [Google Scholar]
  14. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: ASM Press; 2007; pp.330–393
    [Google Scholar]
  15. Kuykendall LD, Roy MA, O'Neill JJ, Devine TE. Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 1988;38:358–361 [CrossRef]
    [Google Scholar]
  16. Fang MX, Zhang WW, Zhang YZ, Tan HQ, Zhang XQ et al. Brassicibacter mesophilus gen. nov., sp. nov., a strictly anaerobic bacterium isolated from food industry wastewater. Int J Syst Evol Microbiol 2012;62:3018–3023 [CrossRef][PubMed]
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  18. Kim SB, Falconer C, Williams E, Goodfellow M. Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 1998;48:59–68 [CrossRef][PubMed]
    [Google Scholar]
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  20. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004;32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  21. Yarza P, Richter M, Peplies J, Euzeby J, Amann R et al. The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 2008;31:241–250 [CrossRef][PubMed]
    [Google Scholar]
  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  23. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  24. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  25. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  26. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  27. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  28. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ et al. ABySS: a parallel assembler for short read sequence data. Genome Res 2009;19:1117–1123 [CrossRef][PubMed]
    [Google Scholar]
  29. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015;25:1043–1055 [CrossRef][PubMed]
    [Google Scholar]
  30. Sun C, Wang RJ, Su Y, Fu GY, Zhao Z et al. Hyphobacterium vulgare gen. nov., sp. nov., a novel alphaproteobacterium isolated from seawater. Int J Syst Evol Microbiol 2017;67:1169–1176 [CrossRef][PubMed]
    [Google Scholar]
  31. Yu XY, Zhai JY, Fu GY, Shen X, Zhao Z et al. Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat. Curr Microbiol 2017;74:1469–1476 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002795
Loading
/content/journal/ijsem/10.1099/ijsem.0.002795
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error