1887

Abstract

A Gram-reaction-negative, S-shaped, motile, poly-β-hydroxybutyrate-accumulating, facultatively anaerobic, beige-pigmented bacterium, designated strain KMU-80, was isolated from seawater collected from the Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family , of the class , and that it possessed the greatest sequence similarity (96.7 %) to NBRC 13613. The DNA G+C content of KMU-80 was 48.3 mol%, and ubiquinone 10 was the sole respiratory quinone. The predominant cellular fatty acids consisted of C 7 (60.2 %), C (13.4 %) and C 7 and/or C 6 (11.1 %). Strain KMU-80 had phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and four unidentified lipids as polar lipids. Based on its distinct phylogenetic position and the combination of genotypic and phenotypic characteristics, this strain is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is KMU-80 (= KCCM 90274 = NBRC 112846). An amended description of the genus is also provided.

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2018-06-01
2020-01-27
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References

  1. Satomi M, Kimura B, Hamada T, Harayama S, Fujii T. Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. no. Int J Syst Evol Microbiol 2002;52:739–747 [CrossRef][PubMed]
    [Google Scholar]
  2. Terasaki Y. Studies on the genus Spirillum Ehrenberg. II. Comments on type and reference strains of Spirillum and description of new species and subspecies. Bull Suzugamine Women’s Coll Nat Sci 1973;17:1–71
    [Google Scholar]
  3. Han SB, Su Y, Hu J, Wang RJ, Sun C et al. Terasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella. Int J Syst Evol Microbiol 2016;66:1807–1812 [CrossRef][PubMed]
    [Google Scholar]
  4. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  9. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  11. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  12. Yoon J, Lee KC, Lee JS. Cribrihabitans pelagius sp. nov., a marine alphaproteobacterium isolated from seawater. Int J Syst Evol Microbiol 2016;66:3195–3200 [CrossRef][PubMed]
    [Google Scholar]
  13. Hertel C, Schmidt G, Fischer M, Oellers K, Hammes WP. Oxygen-dependent regulation of the expression of the catalase gene katA of Lactobacillus sakei LTH677. Appl Environ Microbiol 1998;64:1359–1365[PubMed]
    [Google Scholar]
  14. Høvik Hansen G, Sørheim R. Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 1991;13:231–241 [CrossRef]
    [Google Scholar]
  15. Power DA, Johnson JA. Difco™ and BBL™ Manual: Manual of Microbiological Culture Media, 2nd ed. Sparks: Becton Dickinson and Company; 2009; pp.359–360
    [Google Scholar]
  16. Lewin RA, Lounsbery DM. Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 1969;58:145–170 [CrossRef][PubMed]
    [Google Scholar]
  17. Ostle AG, Holt JG. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol 1982;44:238–241[PubMed]
    [Google Scholar]
  18. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  19. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  20. Komagata K, Suzuki K. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[Crossref]
    [Google Scholar]
  21. Worliczek HL, Kämpfer P, Rosengarten R, Tindall BJ, Busse HJ. Polar lipid and fatty acid profiles–re-vitalizing old approaches as a modern tool for the classification of mycoplasmas?. Syst Appl Microbiol 2007;30:355–370 [CrossRef][PubMed]
    [Google Scholar]
  22. Collins MD, Jones D. A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 1981;51:129–134 [CrossRef][PubMed]
    [Google Scholar]
  23. Terasaki Y. Transfer of five species and two subspecies of Spirillum to other genera (Aquaspirillum and Oceanospirillum), with emended descriptions of the species and subspecies. Int J Syst Evol Microbiol 1979;29:130–144
    [Google Scholar]
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