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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 6

sp. nov., a novel member of isolated from sawdust in a pigpen Free

Abstract

A Gram-stain-negative, rod-shaped, catalase- and oxidase-positive, facultatively anaerobic, and motile bacterium, designated strain SZDIS-1, was isolated from pigpen sawdust bedding in Xiamen, Fujian Province, China. Cells grew at 10–50 °C, pH 6.0–9.0, up to 12 % (w/v) NaCl, resisted vibriostatic agent O/129 and were negative for gelatin and alginate hydrolysis. No growth on thiosulfate citrate bile salts sucrose agar medium. Based on 16S rRNA gene sequences and multilocus sequence analysis, this strain should be assigned to the genus , with the closest relatives being CA-1004 (97.7 % 16S rRNA gene sequence pairwise similarity), SA2 (96.6 %), WS 4539 (96.3 %), S-1 (96.1 %) and MANO22D (95.5 %), but separate from them by large distances in different phylogenetic trees. Based on whole genome analysis, the orthologous average nucleotide identity and DNA–DNA hybridization values against the five relatives were 76.1–78.7 and 20.1–28.7 %. The major fatty acids were summed feature 3 (C 7/C 6), C, summed feature 2 (one or more of C aldehyde, C 3OH and/or iso-C) and summed feature 8 (Cω6 and/or Cω7). The DNA G+C content was 43.0 mol% from whole genomic sequence analysis. Therefore, phylogenetic, genotypic, phenotypic and chemotaxonomic characteristics showed that the isolate represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SZDIS-1 (=DSM 104291=CGMCC 1.15236).

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Keyword(s): chemotaxonomy , genotype , phylogenetics , physiology , polyphasic taxonomy and Vibrio gangliei sp. nov.
© 2018 IUMS
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2018-06-01
2024-04-25
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Vibrio gangliei sp. nov., a novel member of Vibrionaceae isolated from sawdust in a pigpen
Int J Syst Evol Microbiol 68, 1969 (2018); https://doi.org/10.1099/ijsem.0.002779
/content/journal/ijsem/10.1099/ijsem.0.002779
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