1887

Abstract

A novel Gram-stain-negative, aerobic, non-spore-forming, motile and rod-shaped bacterial strain, designated HM451, was isolated from forest soil sampled at the Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31′ E 23° 10′ N). It grew optimally at 28 °C, pH 5.0–6.0 and in the presence of 0–2.5 % (w/v) NaCl on R2A medium. Strain HM451 was closely related to NBRC 106338 (98.6 % 16S rRNA gene sequence similarity), NBRC 101817 (98.4 %) and SRMrh-20 (98.0 %). The 16S rRNA gene sequence analysis showed that strain HM451 and the three closely related strains formed a clade within the genus , but was clearly separated from the established species. The DNA–DNA relatedness value between strain HM451 and its phylogenetically closest relative, NBRC 106338, was much lower than 70 %. Strain HM451 contained ubiquinone 8 as the major respiratory quinone. Major fatty acids were C, Ccyclo and summed feature 8 (Cω7 and/or C 6). The DNA G+C content of strain HM451 was 65.4 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids, one unidentified aminolipid and a polar lipid. The phenotypic, chemotaxonomic and phylogenetic data showed that strain HM451 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HM451 (=GDMCC 1.1190=LMG 30092).

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2018-06-01
2024-03-28
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