1887

Abstract

During a survey of the yeast community associated with the phylloplane of corn in Thailand, a basidiomycetous yeast strain belonging to the genus was isolated. Analyses of the D1/D2 domains of the 26S (LSU) rRNA gene and complete ITS region supported the recognition of a novel species, for which the name sp. nov. is proposed (type strain DMKU-CP801=CBS 15220=PYCC 7257). Another strain of sp. nov., isolated in French Guiana, was found to be sexually compatible with the Thai isolate and mycelium with clamp connections, basidia and basidiospores were observed in culture. The basidial morphology of combined features previously observed for and , which represent the only sexual species hitherto known in the genus, i.e. transversely septate basidia, with sexual structures of the type.

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2018-06-01
2024-03-29
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References

  1. Sampaio JP, Weiß M, Gadanho M, Bauer R. New taxa in the Tremellales: Bulleribasidium oberjochense gen. et sp. nov., Papiliotrema bandonii gen. et sp. nov. and Fibulobasidium murrhardtense sp. nov. Mycologia 2002; 94:873–887[PubMed]
    [Google Scholar]
  2. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV et al. Towards an integrated phylogenetic classification of the Tremellomycetes . Stud Mycol 2015; 81:85–147 [View Article][PubMed]
    [Google Scholar]
  3. Sampaio JP, Inácio J, Fonseca A, Gadanho M, Spencer-Martins I et al. Auriculibuller fuscus gen. nov., sp. nov. and Bullera japonica sp. nov., novel taxa in the Tremellales. Int J Syst Evol Microbiol 2004; 54:987–993 [View Article][PubMed]
    [Google Scholar]
  4. Surussawadee J, Khunnamwong P, Srisuk N, Limtong S. Papiliotrema siamense f.a., sp. nov., a yeast species isolated from plant leaves. Int J Syst Evol Microbiol 2014; 64:3058–3062 [View Article][PubMed]
    [Google Scholar]
  5. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article][PubMed]
    [Google Scholar]
  6. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990 pp. 315–322
    [Google Scholar]
  7. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  9. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts, A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp. 87–110 [Crossref]
    [Google Scholar]
  10. Liu XZ, Wang QM, Theelen B, Groenewald M, Bai FY. Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses. Stud Mycol 2015; 81:1–26 [View Article][PubMed]
    [Google Scholar]
  11. Yurkov A, Guerreiro MA, Sharma L, Carvalho C, Fonseca Á. Multigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. terrestris (Tremellales). PLoS One 2015; 10:e0120400 [View Article][PubMed]
    [Google Scholar]
  12. Sylvester K, Wang QM, James B, Mendez R, Hulfachor AB et al. Temperature and host preferences drive the diversification of Saccharomyces and other yeasts: a survey and the discovery of eight new yeast species. FEMS Yeast Res 2015; 15:1–16 [View Article][PubMed]
    [Google Scholar]
  13. Machado Pagani D, Brandão LR, Santos AR, Felix CR, Pais Ramos J et al. Papiliotrema leoncinii sp. nov. and Papiliotrema miconiae sp. nov., two tremellaceous yeast species from Brazil. Int J Syst Evol Microbiol 2016; 66:1799–1806 [View Article][PubMed]
    [Google Scholar]
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