A novel orange-pigmented, Gram-stain-negative and strictly aerobic bacterium, designated strain HR-UT, was isolated from a water sample of the Han River located in the Republic of Korea. Cells were catalase-positive and oxidase-positive, and non-motile curved rods without flagella. The strain grew at 5–35 °C (optimum 25 °C), pH 6–10 (optimum of 7–8) and 0–3 % (w/v) NaCl (optimum 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HR-UT formed a tight phylogenic lineage with Siphonobacter aquaeclarae DSM 21668T and ‘Siphonobacter intestinalis’ 63MJ-2. The 16S rRNA gene sequence comparison of strain HR-UT and other reported type strains showed that it shared the highest sequence similarity to S. aquaeclarae DSM 21668T (96.9 %) and ‘S. intestinalis’ 63MJ-2 (96.6 %), and had lower similarities than 87.0 % to other bacteria with validly published names. Average nucleotide identity and in silico DNA–DNA hybridization values between strain HR-UT and the most closely related strain S. aquaeclarae were 71.7 and 18.4 %, respectively. The major respiratory quinone was menaquinone-7, and the dominant fatty acids (>5 %) consisted of C16 : 1ω5c, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C15 : 0. The polar lipids comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids. The DNA G+C content was 47.9 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain HR-UT represents a novel species of the genus Siphonobacter, for which the name Siphonobacter curvatus sp. nov. is proposed. The type strain is HR-UT (=KACC 19409T=JCM 32267T).
TáncsicsA,
KékiZ,
MárialigetiK,
SchumannP,
TóthEM.
Siphonobacter aquaeclarae gen. nov., sp. nov., a novel member of the family 'Flexibacteraceae', phylum Bacteroidetes
. Int J Syst Evol Microbiol2010; 60:2567–2571 [View Article][PubMed]
LeeSA,
KimJM,
AhnJH,
JoaJH,
KimSJ et al.Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus
. J Microbiol2016; 54:709–712 [View Article][PubMed]
LeeY,
JeonCO.
Sphingomonas frigidaeris sp. nov., isolated from an air conditioning system. Int J Syst Evol Microbiol2017; 67:3907–3912 [View Article][PubMed]
YoonSH,
HaSM,
KwonS,
LimJ,
KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617 [View Article][PubMed]
StamatakisA.
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics2014; 30:1312–1313 [View Article][PubMed]
LeeI,
Ouk KimY,
ParkSC,
ChunJ.
OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol2016; 66:1100–1103 [View Article][PubMed]
StackebrandtE,
FrederiksenW,
GarrityGM,
GrimontPA,
KämpferP et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol2002; 52:1043–1047 [View Article][PubMed]
RichterM,
Rosselló-MóraR.
Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA2009; 106:19126–19131 [View Article][PubMed]
SmibertRM,
KriegNR.
Phenotypic characterization. In
GerhardtP.
(editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
MinnikinDE,
PatelPV,
AlshamaonyL,
GoodfellowM.
Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol1977; 27:104–117 [View Article]