1887

Abstract

A novel actinomycete isolate, designated strain MK44, was isolated from a Manganese-polluted soil sample collected near Xiangtan Manganese Mine, South Central China and subjected to a polyphasic taxonomic characterization. Comparison of 16S rRNA gene sequences showed that strain MK44 was a member of the genus and most closely related to JCM 16611 (97.9 %) and JCM 16957 (97.4 %). The DNA–DNA relatedness between strain MK44 and the above two related type species were 30.9±0.3 and 29.9±3.5 %, respectively, values which are far lower than the 70 % threshold for the delineation of a novel prokaryotic species. Furthermore, the results of physiological, biochemical and chemotaxonomic tests allowed further phenotypic differentiation. Therefore, it is concluded that strain MK44 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MK44 (=GDMCC 4.137=KCTC 39920).

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2018-06-01
2024-12-03
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References

  1. Bou Kheir R, Shomar B, Greve MB, Greve MH. On the quantitative relationships between environmental parameters and heavy metals pollution in Mediterranean soils using GIS regression-trees: The case study of Lebanon. J Geochem Explor 2014; 147:250–259 [View Article]
    [Google Scholar]
  2. Ling W, Shen Q, Gao Y, Gu X, Yang Z. Use of bentonite to control the release of copper from contaminated soils. Soil Res 2007; 45:618–623 [View Article]
    [Google Scholar]
  3. Umrania VV. Bioremediation of toxic heavy metals using acidothermophilic autotrophes. Bioresour Technol 2006; 97:1237–1242 [View Article][PubMed]
    [Google Scholar]
  4. Wang C, Li Y, Liu Z, Wang P. Bioremediation of nitrobenzene-polluted sediments by Pseudomonas putida . Bull Environ Contam Toxicol 2009; 83:865–868 [View Article][PubMed]
    [Google Scholar]
  5. Lu J, Wu J, Chen T, Wilson PC, Qian J et al. Valuable metal recovery during the bioremediation of acidic mine drainage using sulfate reducing straw bioremediation system. Water Air Soil Pollut 2012; 223:3049–3055 [View Article]
    [Google Scholar]
  6. Yang Z, Zhang Z, Chai L, Wang Y, Liu Y et al. Bioleaching remediation of heavy metal-contaminated soils using Burkholderia sp. Z-90. J Hazard Mater 2016; 301:145–152 [View Article][PubMed]
    [Google Scholar]
  7. Habibul N, Hu Y, Sheng GP. Microbial fuel cell driving electrokinetic remediation of toxic metal contaminated soils. J Hazard Mater 2016; 318:9–14 [View Article][PubMed]
    [Google Scholar]
  8. Albarracín VH, Avila AL, Amoroso MJ, Abate CM. Copper removal ability by Streptomyces strains with dissimilar growth patterns and endowed with cupric reductase activity. FEMS Microbiol Lett 2008; 288:141–148 [View Article][PubMed]
    [Google Scholar]
  9. Albarracín VH, Winik B, Kothe E, Amoroso MJ, Abate CM. Copper bioaccumulation by the actinobacterium Amycolatopsis sp. AB0. J Basic Microbiol 2008; 48:323–330 [View Article][PubMed]
    [Google Scholar]
  10. Amoroso MJ, Castro GR, Durán A, Peraud O, Oliver G et al. Chromium accumulation by two Streptomyces spp. isolated from riverine sediments. J Ind Microbiol Biotechnol 2001; 26:210–215 [View Article][PubMed]
    [Google Scholar]
  11. Benimeli CS, Fuentes MS, Abate CM, Amoroso MJ. Bioremediation of lindane-contaminated soil by Streptomyces sp. M7 and its effects on Zea mays growth. Int Biodeterior Biodegradation 2008; 61:233–239 [View Article]
    [Google Scholar]
  12. Desjardin V. Utilisation of supernatants of pure cultures of Streptomyces thermocarboxydus NH50 to reduce chromium toxicity and mobility in contaminated soils. Water Air Soil Pollut 2003; 3:153–160 [View Article]
    [Google Scholar]
  13. Siñeriz ML, Kothe E, Abate CM. Cadmium biosorption by Streptomyces sp. F4 isolated from former uranium mine. J Basic Microbiol 2009; 49:S55–S62 [View Article][PubMed]
    [Google Scholar]
  14. Sineva ON, Terekhova LP. Selective Isolation of Rare Actinomycetes from Soil. Antibiot Khimioter 2015; 60:27–33[PubMed]
    [Google Scholar]
  15. Peng YX, Jiang Y, Duan SR, WJ L, LH X. Selective isolation methods of rare actinomycetes. J Yunnan Univ 2007; 29:86–89
    [Google Scholar]
  16. Atlas RM. Handbook of Microbiological Media In Parks LC. (editor) Boca Raton, FL: CRC Press; 1993
    [Google Scholar]
  17. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
    [Google Scholar]
  18. Dong XZ, Cai MY. Manual of Systematic and Identification of General Beijing: Science Press; 2001
    [Google Scholar]
  19. Ridgway R. Color Standards and Color Nomenclature Washington, DC: 1912 pp. 1–43 plate I–LII [Crossref]
    [Google Scholar]
  20. Shieh WY, Chen YW, Chaw SM, Chiu HH. Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 2003; 53:479–484 [View Article][PubMed]
    [Google Scholar]
  21. Xu LH, Li WJ, Liu ZH, Jiang CL. Actinomycete Systematic-Principle, Methods and Practice Beijing: Science press; 2007
    [Google Scholar]
  22. Ruan JS, Huang Y. Rapid Identification and Systematics of Actinobacteria Beijing: Science press; 2011
    [Google Scholar]
  23. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [View Article]
    [Google Scholar]
  24. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970; 20:435–443 [View Article]
    [Google Scholar]
  25. MIDI Sherlock Microbial Identification System Operating Manual, Version 6.0 Newark DE: MIDI Inc; 2005
    [Google Scholar]
  26. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  27. Kates M. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986
    [Google Scholar]
  28. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  29. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: Wiley; 1991 pp. 115–175
    [Google Scholar]
  30. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  31. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  32. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  33. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  34. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  35. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
    [Google Scholar]
  36. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
    [Google Scholar]
  37. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
    [Google Scholar]
  38. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O et al. International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [Crossref]
    [Google Scholar]
  39. Wright F, Bibb MJ. Codon usage in the G+C-rich Streptomyces genome. Gene 1992; 113:55–65 [View Article][PubMed]
    [Google Scholar]
  40. Kämpfer P, Huber B, Buczolits S, Thummes K, Grün-Wollny I et al. Streptomyces specialis sp. nov. Int J Syst Evol Microbiol 2008; 58:2602–2606 [View Article][PubMed]
    [Google Scholar]
  41. Chen HH, Qin S, Lee JC, Kim CJ, Xu LH et al. Streptomyces mayteni sp. nov., a novel actinomycete isolated from a Chinese medicinal plant. Antonie van Leeuwenhoek 2009; 95:47–53 [View Article][PubMed]
    [Google Scholar]
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