1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 6

gen. nov., sp. nov., isolated from a marine solar saltern Free

Abstract

A halophilic archaeal strain, designated ZS-35-S2, was isolated from Zhoushan marine solar saltern in Zhejiang Province, China. Cells were pleomorphic, Gram-stain-negative and formed red-pigmented colonies on agar plates. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Strain ZS-35-S2 was able to grow at 25–50 °C (optimum, 37 °C), with 1.4–4.8 M NaCl (optimum, 2.1 M), with 0–1.0 M MgCl (optimum, 0.1 M) and at pH 5.0–9.5 (optimum, pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA genes and genes revealed that strain ZS-35-S2 was distinct from the related genera , , , and of the order . The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three unidentified glycolipids. The two major glycolipids were chromatographically identical to S-DGD-1 and DGD-1, respectively. The DNA G+C content of strain ZS-35-S2 is 67.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-35-S2 (=CGMCC 1.12551=JCM 30036) represents a novel species of a new genus within the order , for which the name gen. nov., sp. nov. is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): halophilic archaeon , marine solar saltern and Salinirubellus salinus gen. nov., sp. nov.
© 2018 IUMS
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2018-06-01
2024-04-19
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References

  1. Grant WD, Kamekura M, McGenity TJ, Ventosa A. Class III. Halobacteria class. nov. In Boone DR, Castenholz RW, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 1 New York: Springer; 2001 pp. 294–334
     [Google Scholar]
  2. Chen S, Xu Y, Ke LX. Halorubrum trueperi sp. nov., a halophilic archaeon isolated from a salt mine. Int J Syst Evol Microbiol 2017; 67:1564–1570 [View Article][PubMed]
     [Google Scholar]
  3. Rasooli M, Naghoni A, Amoozegar MA, Mirfeizi L, Moshtaghi Nikou M et al. Natrinema soli sp. nov., a novel halophilic archaeon isolated from a hypersaline wetland. Int J Syst Evol Microbiol 2017; 67:2142–2147 [View Article][PubMed]
     [Google Scholar]
  4. Sorokin DY, Messina E, Smedile F, Roman P, Damsté JSS et al. Discovery of anaerobic lithoheterotrophic haloarchaea, ubiquitous in hypersaline habitats. ISME J 2017; 11:1245–1260 [View Article][PubMed]
     [Google Scholar]
  5. Xu WM, Xu JQ, Zhou Y, Li Y, Lü Z-Z et al. Halomarina salina sp. nov., isolated from a marine solar saltern. Antonie van Leeuwenhoek 2016; 109:1121–1126 [View Article][PubMed]
     [Google Scholar]
  6. Albuquerque L, Kowalewicz-Kulbat M, Drzewiecka D, Stączek P, D'Auria G et al. Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland. Syst Appl Microbiol 2016; 39:100–105 [View Article][PubMed]
     [Google Scholar]
  7. Sánchez-Nieves R, Facciotti MT, Saavedra-Collado S, Dávila-Santiago L, Rodríguez-Carrero R et al. Draft genome sequence of Halorubrum tropicale strain V5, a novel halophilic archaeon isolated from the solar salterns of Cabo Rojo, Puerto Rico. Genom Data 2016; 7:284–286 [View Article][PubMed]
     [Google Scholar]
  8. McDuff S, King GM, Neupane S, Myers MR. Isolation and characterization of extremely halophilic CO-oxidizing Euryarchaeota from hypersaline cinders, sediments and soils and description of a novel CO oxidizer, Haloferax namakaokahaiae Mke2.3T, sp. nov. FEMS Microbiol Ecol 2016; 92:fiw028 [View Article][PubMed]
     [Google Scholar]
  9. Sánchez-Nieves R, Facciotti M, Saavedra-Collado S, Dávila-Santiago L, Rodríguez-Carrero R et al. Draft genome of Haloarcula rubripromontorii strain SL3, a novel halophilic archaeon isolated from the solar salterns of Cabo Rojo, Puerto Rico. Genom Data 2016; 7:287–289 [View Article][PubMed]
     [Google Scholar]
  10. Chen S, Liu HC, Zhou J, Xiang H. Haloparvum sedimenti gen. nov., sp. nov., a member of the family Haloferacaceae. Int J Syst Evol Microbiol 2016; 66:2327–2334 [View Article][PubMed]
     [Google Scholar]
  11. Mori K, Nurcahyanto DA, Kawasaki H, Lisdiyanti P, Yopi et al. Haloarchaeobius baliensis sp. nov., isolated from a solar saltern. Int J Syst Evol Microbiol 2016; 66:38–43 [View Article][PubMed]
     [Google Scholar]
  12. Chen S, Liu HC, Zhao D, Yang J, Zhou J et al. Halorubrum yunnanense sp. nov., isolated from a subterranean salt mine. Int J Syst Evol Microbiol 2015; 65:4526–4532 [View Article][PubMed]
     [Google Scholar]
  13. Chen S, Liu HC, Zhou J, Xiang H. Halorubrum pallidum sp. nov., an extremely halophilic archaeon isolated from a subterranean rock salt. Int J Syst Evol Microbiol 2016; 66:2980–2986 [View Article][PubMed]
     [Google Scholar]
  14. Jaakkola ST, Pfeiffer F, Ravantti JJ, Guo Q, Liu Y et al. The complete genome of a viable archaeum isolated from 123-million-year-old rock salt. Environ Microbiol 2016; 18:565–579 [View Article][PubMed]
     [Google Scholar]
  15. Oren A, Ventosa A, Grant WD. Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 1997; 47:233–238 [View Article]
     [Google Scholar]
  16. Mou YZ, Qiu XX, Zhao ML, Cui HL, Oh D et al. Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively. Extremophiles 2012; 16:895–901 [View Article][PubMed]
     [Google Scholar]
  17. Cui HL, Gao X, Yang X, Xu XW. Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 2010; 14:493–499 [View Article][PubMed]
     [Google Scholar]
  18. Kates M. Separation of lipid mixtures. Techniques of Lipidology, 2nd ed. Amsterdam: Elsevier; 1986 pp. 241–246
     [Google Scholar]
  19. Cui HL, Zhou PJ, Oren A, Liu SJ. Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 2009; 13:31–37 [View Article][PubMed]
     [Google Scholar]
  20. Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R et al. Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B' (rpoB') gene. Int J Syst Evol Microbiol 2010; 60:2398–2408 [View Article][PubMed]
     [Google Scholar]
  21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  22. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
     [Google Scholar]
  23. Kim TY, Kim SJ, Park SJ, Kim JG, Cha IT et al. Natronomonas gomsonensis sp. nov., isolated from a solar saltern. Antonie van Leeuwenhoek 2013; 104:627–635 [View Article][PubMed]
     [Google Scholar]
  24. Inoue K, Itoh T, Ohkuma M, Kogure K. Halomarina oriensis gen. nov., sp. nov., a halophilic archaeon isolated from a seawater aquarium. Int J Syst Evol Microbiol 2011; 61:942–946 [View Article][PubMed]
     [Google Scholar]
  25. Burns DG, Janssen PH, Itoh T, Minegishi H, Usami R et al. Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas. Int J Syst Evol Microbiol 2010; 60:1173–1176 [View Article][PubMed]
     [Google Scholar]
  26. Yuan PP, Yin S, Han D, Zhang WJ, Cui HL. Halorientalis brevis sp. nov., Isolated from an inland salt lake of China. Curr Microbiol 2015; 71:382–386 [View Article][PubMed]
     [Google Scholar]
  27. Cui HL, Qiu XX. Salinarubrum litoreum gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from Chinese marine solar salterns. Antonie van Leeuwenhoek 2014; 105:135–141 [View Article][PubMed]
     [Google Scholar]
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Salinirubellus salinus gen. nov., sp. nov., isolated from a marine solar saltern
Int J Syst Evol Microbiol 68, 1874 (2018); https://doi.org/10.1099/ijsem.0.002757
/content/journal/ijsem/10.1099/ijsem.0.002757
/content/journal/ijsem/10.1099/ijsem.0.002757
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