1887

Abstract

A halophilic archaeal strain, designated ZS-35-S2, was isolated from Zhoushan marine solar saltern in Zhejiang Province, China. Cells were pleomorphic, Gram-stain-negative and formed red-pigmented colonies on agar plates. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Strain ZS-35-S2 was able to grow at 25–50 °C (optimum, 37 °C), with 1.4–4.8 M NaCl (optimum, 2.1 M), with 0–1.0 M MgCl (optimum, 0.1 M) and at pH 5.0–9.5 (optimum, pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA genes and genes revealed that strain ZS-35-S2 was distinct from the related genera , , , and of the order . The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and three unidentified glycolipids. The two major glycolipids were chromatographically identical to S-DGD-1 and DGD-1, respectively. The DNA G+C content of strain ZS-35-S2 is 67.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-35-S2 (=CGMCC 1.12551=JCM 30036) represents a novel species of a new genus within the order , for which the name gen. nov., sp. nov. is proposed.

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2018-06-01
2019-12-15
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