1887

Abstract

A Gram-stain-positive, non-motile, creamy-white and rod-coccus shaped actinobacterium, designated strain Ktm-20, capable of degrading petroleum oil was isolated from oil-contaminated soil. Strain Ktm-20 was able to grow at 15–37 °C, at pH 5.5–10.0 and at 0.0–2.0 % (w/v) NaCl concentration. This strain was taxonomically characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain Ktm-20 belonged to the genus and is closely related to DSM 44892, UC12, NBRC 100605, CFH S0262 and MBRL 353T (98.8, 98.7, 98.5, 98.4 and 98.3 % gene sequence similarity, respectively). The only respiratory quinone was MK-8(H2); the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and the predominant fatty acids were C, summed feature 3 (Cω7 and/or Cω6) and Cω9. The cell-wall peptidoglycan contained meso-diaminopimelic acid; and galactose, glucose, arabinose and ribose were detected as diagnostic sugars from whole-cell hydrolysates. Mycolic acids were detected. The DNA G+C content was 70.9 mol%. The DNA–DNA relatedness values between strain Ktm-20 and closely related species of the genus were between 38.3–25.3 %, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain Ktm-20 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Ktm-20 (=KEMB 9005-695=KACC 19390=JCM 32206).

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2018-05-01
2020-01-21
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