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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 5

Addressing the diversity of the honeybee gut symbiont : description of sp. nov., isolated from the gut of honeybees () Free

Abstract

The gut microbiota of honeybees () and bumblebees () include the symbiotic bacterial genus . This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. wkB1, which was isolated from , was the first species to be described. Recently four new species, isolated from , were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different species. We show that one lineage, isolated only from , is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 %) and phenotypic characterizations. We propose the new species name for this lineage: sp. nov. We present the characterization of the type strain NO3 (=DSM 105629=LMG 30293), a strain isolated from the Western honeybee , which clusters within this lineage. Cells of strain NO3 grow best in a microaerophilic atmosphere with enhanced CO levels at 36 °C and pH 7.0–7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1 µm in length and rod-shaped, and the genomic G+C content is 34.7 mol%. Differential characteristics between strain NO3 and the different type strains of were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3 was ubiquinone-8, and the predominant fatty acids were Cω7c/Cω6c, C, consistent with the genus .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome phylogeny , Gilliamella apis NO3T and honeybee gut symbiont
© 2018 IUMS
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2018-05-01
2024-04-19
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Addressing the diversity of the honeybee gut symbiont Gilliamella: description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)
Int J Syst Evol Microbiol 68, 1762 (2018); https://doi.org/10.1099/ijsem.0.002749
/content/journal/ijsem/10.1099/ijsem.0.002749
/content/journal/ijsem/10.1099/ijsem.0.002749
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