1887

Abstract

A Gram-stain-negative, rod-shaped, aerobic, straw yellow, motile strain, designated KNDSW-TSA6, belonging to the genus , was isolated from a water sample of the river Ganges, downstream of the city of Kanpur, Uttar Pradesh, India. Cells were aerobic, non-endospore-forming and motile with single polar flagella. It differed from its phylogenetically related strains by phenotypic characteristics such as hydrolysis of urea, gelatin, casein and DNA, and the catalase reaction. The major fatty acids were C 7C 6, C and C 7C 6. Phylogenetic analysis based on 16S rRNA and housekeeping genes (, and gene sequences), confirmed its placement within the genus as a novel species. Strain KNDSW-TSA6 showed highest 16S rRNA sequence similarity to BL21 (98.9 %), ATCC 17505 (98.8 %), CCUG 11779 (98.2 %), R-24608 (97.9 %) and N35 (97.6 %). The digital DNA–DNA hybridization and average nucleotide identity values calculated from whole genome sequences between strain KNDSW-TSA6 and the two most closely related strains BL21 and ATCC 17505 were below the threshold values of 70 and 95 % respectively. Thus, the data from the polyphasic taxonomic analysis clearly indicates that strain KNDSW-TSA6 represents a novel species, for which the name sp. nov. is proposed. The type strain is (=MTCC 12652=KCTC 52819=VTCC-B-910010).

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2018-05-01
2024-03-28
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