@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002734, author = "Wu, Yue-Hong and Yan, Jing and Fang, Chen and Huo, Ying-Yi and Ma, Wei-Lin and Zhang, De-Min and Wang, Chun-Sheng and Xu, Xue-Wei", title = "Gracilimonas amylolytica sp. nov., isolated from deep-sea sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2018", volume = "68", number = "5", pages = "1713-1718", doi = "https://doi.org/10.1099/ijsem.0.002734", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002734", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Gracilimonas", keywords = "deep-sea sediment", keywords = "phylogenetic analysis", keywords = "whole genome sequencing", keywords = "Rhodothermaeota", abstract = "A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated LA399T, was isolated from deep-sea sediment collected from the Pacific Ocean. Cells of strain LA399T grew in the medium containing 0–10.0 % of NaCl (w/v; optimum 3.0–5.0 %), pH 6.5–8.0 (optimum 7.0) and 20–40 °C (optimum 37 °C). Aesculin, gelatin, starch and Tween 80 were hydrolysed. Strain LA399T was closely related to Gracilimonas halophila WDS2C40T (97.0 % sequence similarity), Gracilimonas mengyeensis YIM J14T (96.4 %), Gracilimonas rosea CL-KR2T (96.4 %) and Gracilimonas tropica DSM 19535T (96.0 %), and exhibited equal or less than 96.0 % sequence similarity to other type strains of species with validly published names. Phylogenetic analyses revealed that strain LA399T clustered with the clade comprising the Gracilimonas species and formed an independent lineage. Strain LA399T contained menaquinone 7 as the sole isoprenoid quinone and iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c/10-methyl C16 : 0) as the predominant cellular fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and three unidentified glycolipids. The DNA G+C content was 45.3 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Gracilimonas , for which the name Gracilimonas amylolytica is proposed. The type strain is LA399T (=CGMCC 1.16248T=KCTC 52885T).", }