1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 5

sp. nov., isolated from a sediment sample from a lake in India Free

Abstract

An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India. Colonies were circular, 1–2 mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48 h growth on marine agar at pH 9 and 37 °C. Growth occurred at 15–42 °C, 0–10 % (w/v) NaCl and at a pH range of 7–12. Strain AK73 was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, β-galactosidase, lipase (Tween 60) and urease activities. The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C, anteiso-C, C and anteiso-C; MK-7 was the major menaquinone. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids. The DNA G+C content of strain AK73 was 54 mol%. Analysis based on comparative 16S rRNA gene sequence analysis indicated that was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0 %. Phylogenetic analysis showed that strain AK73 formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained , and with similarity values of 93.6, 93.5 and 93.4 %, respectively. Based on its phenotypic characteristics and on phylogenetic inference, strain AK73 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AK73 (=JCM 32184=MTCC 12637=KCTC 33880).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence based phylogeny , Bacillus alkalilacus and chemotaxonomy
© 2018 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002726
2018-05-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/5/1665.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002726&mimeType=html&fmt=ahah

References

  1. Cohn F. Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen Heft 2 1872; 1:127–224
     [Google Scholar]
  2. Niall A, Logan, De Vos P. Genus I. Bacillus Cohn 1872, 174AL . In Vos PD, Garrity GM, Jones D, Krieg NR, Ludwig W et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 3 The Firmicutes New York: Springer; 2009 pp. 21–127
     [Google Scholar]
  3. Parte AC. LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 2014; 42:D613–D616 [View Article][PubMed]
     [Google Scholar]
  4. Ehrenberg CG. Dritter Beitrag zur Erkenntniss grosser Organisation in der Richtung des kleinsten Raumes. Physikalische Abhandlungen der Koeniglichen Akademie der Wissenschaften zu Berlin aus den Jahren 1833; 1835:143–336
     [Google Scholar]
  5. Vandamme P, Pot B, Gillis M, de Vos P, Kersters K et al. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 1996; 60:407–438[PubMed]
     [Google Scholar]
  6. Logan NA, Berge O, Bishop AH, Busse HJ, de Vos P et al. Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 2009; 59:2114–2121 [View Article][PubMed]
     [Google Scholar]
  7. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  8. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
     [Google Scholar]
  9. Srinivas TN, Nageswara Rao SS, Vishnu Vardhan Reddy P, Pratibha MS, Sailaja B et al. Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of kongsfjorden and Ny-alesund, Svalbard, Arctic. Curr Microbiol 2009; 59:537–547 [View Article][PubMed]
     [Google Scholar]
  10. Anil Kumar P, Aravind R, Francis K, Bhumika V, Ritika C et al. Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family "Cyclobacteriaceae" with nitrate reducing activity. Syst Appl Microbiol 2012; 35:320–325 [View Article][PubMed]
     [Google Scholar]
  11. Baek SH, Cui Y, Kim SC, Cui CH, Yin C et al. Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2011; 61:1715–1719 [View Article][PubMed]
     [Google Scholar]
  12. Sasser M. Identification of bacteria through fatty acid analysis. In Klement Z, Rudolph K, Sands DC. (editors) Methods in Phytobacteriology Hungry, Budapest: Akademiai Kiado; 1990 pp. 199–204
     [Google Scholar]
  13. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  14. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47:1129–1133 [View Article][PubMed]
     [Google Scholar]
  15. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–206 [Crossref]
     [Google Scholar]
  16. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article][PubMed]
     [Google Scholar]
  17. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–218 [View Article]
     [Google Scholar]
  18. Sly LI, Blackall LL, Kraat PC, Tian-Shen T, Sangkhobol V. The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 1986; 5:139–156 [Crossref]
     [Google Scholar]
  19. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Good Fellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp. 115–175
     [Google Scholar]
  20. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  21. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
     [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  25. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  26. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002726
Loading
Bacillus alkalilacus sp. nov., isolated from a sediment sample from a lake in India
Int J Syst Evol Microbiol 68, 1665 (2018); https://doi.org/10.1099/ijsem.0.002726
/content/journal/ijsem/10.1099/ijsem.0.002726
/content/journal/ijsem/10.1099/ijsem.0.002726
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error