1887

Abstract

A halophilic bacterial strain, HL-109, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic and chemotaxonomic data was used to classify the strain within the order The organism stained Gram-negative and was a moderate thermophile with a growth optimum of 45 °C. It was obligately aerobic, heterotrophic and halophilic, growing in both NaCl and MgSO brines. The novel isolate had a polymorphic cellular morphology of short rods with occasional branching, and cells were monotrichous. The major fatty acids detected were C, C, C and C. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order and it shared 94 % identity with the type strain of its nearest relative, . Morphological, chemotaxonomic and phylogenetic results did not affiliate the novel organism with any of the families in the ; therefore, HL-109 is representative of a new lineage, for which the name gen. nov., sp. nov. is proposed, with the type strain HL-109 (=JCM 31876=DSM 102886). In addition, examination of the phylogenetics of strain HL-109 and its nearest relatives, and , demonstrates that these halophiles form a clade distinct from the described families of the . We further propose the establishment of a new family, fam. nov., to accommodate the genera and (the type genus of the family).

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2018-05-01
2024-12-03
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