1887

Abstract

Three actinomycete strains originating from the surface-sterilized roots of were characterized by using a polyphasic approach. Phylogenetic analyses based on the 16S rRNA gene sequence showed that they formed a deep, monophyletic branch in the genus , and were most closely related to the type strains of the species and Morphological and chemotaxonomic data supported the affiliation of strains CPCC 204357, CPCC 204354 and CPCC 204355 to the genus . The results of physiological and biochemical tests allowed phenotypic differentiation of strains CPCC 204357, CPCC 204354 and CPCC 204355 from their closest phylogenetic related species in the genus . Low levels of DNA–DNA relatedness with its closest type strains of and indicated that strain CPCC 204357 represent a novel species, for which the name sp. nov. is proposed, with CPCC 204357 (=DSM 102295=KCTC 39745) as the type strain.

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2018-05-01
2024-04-23
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References

  1. Labeda DP, Testa RT, Lechevalier MP, Lechevalier HA. Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol 1985; 35:417–421 [View Article]
    [Google Scholar]
  2. Han XX, Luo XX, Zhang LL. Glycomyces fuscus sp. nov. and Glycomyces albus sp. nov., actinomycetes isolated from a hypersaline habitat. Int J Syst Evol Microbiol 2014; 64:2437–2441 [View Article][PubMed]
    [Google Scholar]
  3. Labeda DP, Kroppenstedt RM. Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 2004; 54:2343–2346 [View Article][PubMed]
    [Google Scholar]
  4. Guan TW, Xia ZF, Xiao J, Wu N, Chen ZJ et al. Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China. Antonie van Leeuwenhoek 2011; 100:137–143 [View Article][PubMed]
    [Google Scholar]
  5. Guan TW, Wang PH, Tian L, Tang SK, Xiang HP. Glycomyces lacisalsi sp. nov., an actinomycete isolated from a hypersaline habitat. Int J Syst Evol Microbiol 2016; 66:5366–5370 [View Article][PubMed]
    [Google Scholar]
  6. Li W, Zhao J, Shi L, Wang J, Wang H et al. Glycomyces rhizosphaerae sp. nov., isolated from the root and rhizosphere soil of wheat (Triticum aestivum L.). Int J Syst Evol Microbiol 2018; 68:223–227 [View Article][PubMed]
    [Google Scholar]
  7. Lv LL, Zhang YF, Zhang LL. Glycomyces tarimensis sp. nov., an actinomycete isolated from a saline-alkali habitat. Int J Syst Evol Microbiol 2015; 65:1587–1591 [View Article][PubMed]
    [Google Scholar]
  8. Evtushenko LI, Taptykova SD, Akimov VN, Semyonova SA, Kalakoutskii LV. Glycomyces tenuis sp. nov. Int J Syst Bacteriol 1991; 41:154–157 [View Article]
    [Google Scholar]
  9. Li W, Liu C, Guo X, Song W, Sun T et al. Glycomyces tritici sp. nov., isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Glycomyces. Antonie van Leeuwenhoek 2017; 12:1–7 [View Article][PubMed]
    [Google Scholar]
  10. Guan TW, Xiang HP, Wang PH, Tian L, Tang SK et al. Glycomyces xinjiangensis sp. nov., a novel actinomycete isolated from a hypersaline habitat. Arch Microbiol 2017; 199:1231–1235 [View Article][PubMed]
    [Google Scholar]
  11. Zhang X, Ren K, Du J, Liu H, Zhang L. Glycomyces artemisiae sp. nov., an endophytic actinomycete isolated from the roots of Artemisia argyi. Int J Syst Evol Microbiol 2014; 64:3492–3495 [View Article][PubMed]
    [Google Scholar]
  12. Qin S, Wang HB, Chen HH, Zhang YQ, Jiang CL et al. Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 2008; 58:2525–2528 [View Article][PubMed]
    [Google Scholar]
  13. Qin S, Chen HH, Klenk HP, Zhao GZ, Li J et al. Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China. Int J Syst Evol Microbiol 2009; 59:1023–1027 [View Article][PubMed]
    [Google Scholar]
  14. Xing K, Qin S, Zhang WD, Cao CL, Ruan JS et al. Glycomyces phytohabitans sp. nov., a novel endophytic actinomycete isolated from the coastal halophyte in Jiangsu, East China. J Antibiot 2014; 67:559–563 [View Article][PubMed]
    [Google Scholar]
  15. Gu Q, Zheng W, Huang Y. Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall. Int J Syst Evol Microbiol 2007; 57:1995–1998 [View Article][PubMed]
    [Google Scholar]
  16. Negi JS, Bisht VK, Bhandari AK, Bhatt VP, Singh P et al. Paris polyphylla: chemical and biological prospectives. Anticancer Agents Med Chem 2014; 14:833–839 [View Article][PubMed]
    [Google Scholar]
  17. Coombs JT, Franco CM. Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 2003; 69:5603–5608 [View Article][PubMed]
    [Google Scholar]
  18. Gu Q, Luo H, Zheng W, Liu Z, Huang Y. Pseudonocardia oroxyli sp. nov., a novel actinomycete isolated from surface-sterilized Oroxylum indicum root. Int J Syst Evol Microbiol 2006; 56:2193–2197 [View Article][PubMed]
    [Google Scholar]
  19. Zhou ZH, Liu ZH, Qian YD, Kim SB, Goodfellow M. Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 1998; 48:53–58 [View Article][PubMed]
    [Google Scholar]
  20. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  21. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983; 129:1743–1813 [View Article][PubMed]
    [Google Scholar]
  22. Zhang YQ, Yu LY, Wang D, Liu HY, Sun CH et al. Roseomonas vinacea sp. nov., a Gram-negative coccobacillus isolated from a soil sample. Int J Syst Evol Microbiol 2008; 58:2070–2074 [View Article][PubMed]
    [Google Scholar]
  23. Yuan LJ, Zhang YQ, Guan Y, Wei YZ, Li QP et al. Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 2008; 58:1180–1185 [View Article][PubMed]
    [Google Scholar]
  24. Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R et al. Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 2007; 57:1424–1428 [View Article][PubMed]
    [Google Scholar]
  25. Thompson JD, Gibson TJ, Higgins DG. Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinformatics 2002; Chapter 2:Unit 2.3 [View Article][PubMed]
    [Google Scholar]
  26. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  27. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  28. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  29. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 2004; 101:11030–11035 [View Article][PubMed]
    [Google Scholar]
  30. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  31. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
    [Google Scholar]
  32. De Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
    [Google Scholar]
  33. Wayne LG. International Committee on Systematic Bacteriology: announcement of the report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Zentralbl Bakteriol Mikrobiol Hyg 1988; 268:433–434
    [Google Scholar]
  34. Stackebrandt E, Goebel BM. Taxonomic Note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  35. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231[PubMed]
    [Google Scholar]
  36. Embley TM, Goodfellow M, Minnikin DE, O'Donnell AG. Lipid and wall amino acid composition in the classification of Rothiadentocariosa. Zentralbl Bakteriol Mikrobiol Hyg 1984; 257:285–295
    [Google Scholar]
  37. Collins MD, Goodfellow M, Minnikin DE. Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curtobacterium and related taxa. J Gen Microbiol 1980; 118:29–37 [View Article][PubMed]
    [Google Scholar]
  38. Guo L, Tuo L, Habden X, Zhang Y, Liu J et al. Allosalinactinospora lopnorensis gen. nov., sp. nov., a new member of the family Nocardiopsaceae isolated from soil. Int J Syst Evol Microbiol 2015; 65:206–213 [View Article][PubMed]
    [Google Scholar]
  39. Kellogg JA, Bankert DA, Withers GS, Sweimler W, Kiehn TE et al. Application of the Sherlock Mycobacteria Identification System using high-performance liquid chromatography in a clinical laboratory. J Clin Microbiol 2001; 39:964–970 [View Article][PubMed]
    [Google Scholar]
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