1887

Abstract

Aplanobacter agropyri was first described in 1915 by O’Gara and later transferred to the genus Corynebacterium by Burkholder in 1948 but it was not included in the Approved Lists of Bacterial Names in 1980 and, consequently, is not recognized as a validly published species. In the 1980s, bacteria resembling Corynebacterium agropyri were isolated from plant samples stored at the Washington State Mycological Herbarium and from a diseased wheatgrass plant collected in Cardwell, Montana, USA. In the framework of this study, eight additional isolates were recovered from the same herbarium plant samples in 2011. The isolates are slow-growing, yellow-pigmented, Gram-stain-positive and coryneform. The peptidoglycan is type B2γ containing diaminobutyric acid, alanine, glycine and glutamic acid, the cell-wall sugars are rhamnose and mannose, the major respiratory quinone is MK-10, and the major fatty acids are anteiso-15 : 0, anteiso 17 : 0 and iso-16 : 0, all of which are typical of the genus Rathayibacter . Phylogenetic analysis of 16S rRNA gene sequences placed the strains in the genus Rathayibacter and distinguished them from the six other described species of Rathayibacter . DNA–DNA hybridization confirmed that the strains were members of a novel species. Based on phenotypic, chemotaxonomic and phylogenetic characterization, it appears that strains CA-1 to CA-12 represent a novel species, and the name Rathayibacter agropyri (non O’Gara 1916) comb. nov., nom. rev. is proposed; the type strain is CA-4 (=DSM 104101;=ATCC TSD-78).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002708
2018-03-20
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/5/1519.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002708&mimeType=html&fmt=ahah

References

  1. Collins MD, Bradbury JF. Plant pathogenic species of Corynebacterium. In Sneath PHA, Mair NS, Sharpe ME, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 2 Baltimore, MD: The Williams & Wilkins Co; 1986; pp.1276–1284
    [Google Scholar]
  2. Davis MJ, Gillaspie AG, Vidaver AK, Harris RW. Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease. Int J Syst Bacteriol 1984;34:107–117 [CrossRef]
    [Google Scholar]
  3. Bousfield IJ, Smith GL, Dando TR, Hobbs G. Numerical analysis of total fatty acid profiles in the identification of coryneform, nocardioform and some other bacteria. Microbiology 1983;129:375–394 [CrossRef]
    [Google Scholar]
  4. Carlson RR, Vidaver AK. Taxonomy of Corynebacterium plant pathogens, including a new pathogen of wheat, based on polyacrylamide gel electrophoresis of cellular proteins. Int J Syst Bacteriol 1982;32:315–326 [CrossRef]
    [Google Scholar]
  5. Collins MD. Cell wall peptidoglycan and lipid composition of the phytopathogen Corynebacterium rathayi (smith). Syst Appl Microbiol 1983;4:193–198 [CrossRef][PubMed]
    [Google Scholar]
  6. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 1980;48:459–470 [CrossRef]
    [Google Scholar]
  7. Döpfer H, Stackebrandt E, Fiedler F. Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa. J Gen Microbiol 1982;128:1697–1708 [CrossRef][PubMed]
    [Google Scholar]
  8. Dye DW, Kemp WJ. A taxonomic study of plant pathogenic Corynebacterium species. N ZJ Agric Res 1977;20:563–582 [CrossRef]
    [Google Scholar]
  9. Starr MP, Mandel M, Murata N. The phytopathogenic coryneform bacteria in the light of dna base composition and DNA–DNA segmental homology. J Gen Appl Microbiol 1975;21:13–26 [CrossRef]
    [Google Scholar]
  10. Collins MD, Brown J, Jones D. Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int J Syst Bacteriol 1988;38:45–48 [CrossRef]
    [Google Scholar]
  11. Stackebrandt E, Smida JAN, Collins MD. Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). J Gen Appl Microbiol 1988;34:341–348 [CrossRef]
    [Google Scholar]
  12. Zgurskaya HI, Evtushenko LI, Akimov VN, Kalakoutskii LV. Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. Int J Syst Bacteriol 1993;43:143–149 [CrossRef]
    [Google Scholar]
  13. Sasaki J, Chijimatsu M, Suzuki K. Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov. Int J Syst Bacteriol 1998;48:403–410 [CrossRef][PubMed]
    [Google Scholar]
  14. Dorofeeva LV, Evtushenko LI, Krausova VI, Karpov AV, Subbotin SA et al. Rathayibacter caricis sp. nov. and Rathayibacter festucae sp. nov., isolated from the phyllosphere of Carex sp. and the leaf gall induced by the nematode Anguina graminis on Festuca rubra L., respectively. Int J Syst Evol Microbiol 2002;52:1917–1923 [CrossRef][PubMed]
    [Google Scholar]
  15. Riley IT, Ophel KM. Clavibacter toxicus sp. nov., the bacterium responsible for annual ryegrass toxicity in Australia. Int J Syst Bacteriol 1992;42:64–68 [CrossRef]
    [Google Scholar]
  16. Vasilenko OV, Starodumova IP, Tarlachkov SV, Dorofeeva LV, Avtukh AN et al. Draft genome sequence of "Rathayibacter tanaceti" strain VKM Ac-2596 isolated from Tanacetum vulgare infested by a foliar nematode. Genome Announc 2016;4:e00512-16 [CrossRef][PubMed]
    [Google Scholar]
  17. Starodumova IP, Tarlachkov SV, Prisyazhnaya NV, Dorofeeva LV, Ariskina EV et al. Draft genome sequence of Rathayibacter sp. strain VKM Ac-2630 isolated from leaf gall induced by the knapweed nematode Mesoanguina picridis on Acroptilon repens. Genome Announc 2017;5:e00650-17 [CrossRef][PubMed]
    [Google Scholar]
  18. O’Gara PJ. A bacterial disease of western wheat grass, Agropyron smithiii. Phytopathol 1916;6:341–350
    [Google Scholar]
  19. O'Gara PJ. A bacterial disease of western wheat-grass. First account of the occurrence of a new type of bacterial disease in America. Science 1915;42:616–617 [CrossRef][PubMed]
    [Google Scholar]
  20. Bergey DH, Harrison FC, Breed RS, Hammer BW, Huntoon FM et al. Genus VIII. Phytomonas gen. nov. In Bergey's Manual of Determinative Bacteriology: A Key for the Identification of Organisms of the Class Schizomycetes Baltimore, MD: The Williams & Wilkins Co; 1923; pp.174–193
    [Google Scholar]
  21. Burkholder WH. Family VIII Corynebacteriaceae Lehman and Neumann. In Breed RS, Murray EGD, Hitchens A. (editors) Bergey's Manual of Determinative Bacteriology, 6th ed. Baltimore, MD: The Williams and Wilkins Co; 1948; pp.381–401
    [Google Scholar]
  22. Skerman VBD, Sneath PHA, McGowan V. Approved lists of bacterial names. Int J Syst Evol Microbiol 1980;30:225–420 [CrossRef]
    [Google Scholar]
  23. Murray TD. Isolation of Corynebacterium agropyri from 30- to 40-year-old herbarium specimens of Agropyron species. Plant Disease 1986;70:378–380 [CrossRef]
    [Google Scholar]
  24. Vidaver AK. Synthetic and complex media for the rapid detection of fluorescence of phytopathogenic pseudomonads: effect of the carbon source. Appl Microbiol 1967;15:1523–1524[PubMed]
    [Google Scholar]
  25. Schaad WW. Identification schemes. In Schaad NW. (editor) Laboratory Guide for Identification of Plant Pathogenic Bacteria St. Paul, MN: American Phytopathological Society; 1980; pp.1–11
    [Google Scholar]
  26. Stackebrandt E, Brambilla E, Richert K. Gene sequence phylogenies of the family Microbacteriaceae. Curr Microbiol 2007;55:42–46 [CrossRef][PubMed]
    [Google Scholar]
  27. Zhi XY, Li WJ, Stackebrandt E. An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 2009;59:589–608 [CrossRef][PubMed]
    [Google Scholar]
  28. Lane DJ. 16S/23S rRNA sequencing. In Stakebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics London, UK: Wiley; 1991; pp.115–176
    [Google Scholar]
  29. Fessehaie A, de Boer SH, Lévesque CA. Molecular characterization of DNA encoding 16S-23S rRNA intergenic spacer regions and 16S rRNA of pectolytic Erwinia species. Can J Microbiol 2002;48:387–398 [CrossRef][PubMed]
    [Google Scholar]
  30. Gross DC, Vidaver AK. Bacteriocins of phytopathogenic Corynebacterium species. Can J Microbiol 1979;25:367–374 [CrossRef][PubMed]
    [Google Scholar]
  31. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular, Technical note 101. North Newark, Del: MIDI, Inc; 2001
    [Google Scholar]
  32. Agarkova IV, Vidaver AK, Postnikova EN, Riley IT, Schaad NW. Genetic characterization and diversity of Rathayibacter toxicus. Phytopathology 2006;96:1270–1277 [CrossRef][PubMed]
    [Google Scholar]
  33. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956;178:703 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002708
Loading
/content/journal/ijsem/10.1099/ijsem.0.002708
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error