1887

Abstract

Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross–reactive immune response they elicit against antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to . The isolates were further characterised by sequence analysis of the partial fragments of , and . The genome of the type strain was also elucidated. Gene (16S rRNA, , and ) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel species. We proposed the name sp. nov. for this new species. The type strain is GPK 1020 (=CIP 110823T=ATCC BAA-2758).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002707
2018-05-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/5/1526.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002707&mimeType=html&fmt=ahah

References

  1. van Ingen J, Boeree MJ, Dekhuijzen PN, van Soolingen D. Environmental sources of rapid growing nontuberculous mycobacteria causing disease in humans. Clin Microbiol Infect 2009;15:888–893 [CrossRef][PubMed]
    [Google Scholar]
  2. Mirsaeidi M, Farshidpour M, Allen MB, Ebrahimi G, Falkinham JO. Highlight on advances in nontuberculous mycobacterial disease in North America. Biomed Res Int 2014;2014:1–10 [CrossRef][PubMed]
    [Google Scholar]
  3. Primm TP, Lucero CA, Falkinham JO. Health impacts of environmental mycobacteria. Clin Microbiol Rev 2004;17:98–106 [CrossRef][PubMed]
    [Google Scholar]
  4. Marras TK, Mendelson D, Marchand-Austin A, May K, Jamieson FB. Pulmonary nontuberculous mycobacterial disease, Ontario, Canada, 1998–2010. Emerg Infect Dis 2013;19:1889–1891 [CrossRef][PubMed]
    [Google Scholar]
  5. Lai CC, Tan CK, Chou CH, Hsu HL, Liao CH et al. Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000–2008. Emerg Infect Dis 2010;16:294–296 [CrossRef][PubMed]
    [Google Scholar]
  6. Lee MR, Sheng WH, Hung CC, Yu CJ, Lee LN et al. Mycobacterium abscessus complex infections in humans. Emerg Infect Dis 2015;21:1638 [CrossRef][PubMed]
    [Google Scholar]
  7. Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN et al. Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 2010;57:205–220 [CrossRef][PubMed]
    [Google Scholar]
  8. Geluk A, van Meijgaarden KE, Franken KL, Wieles B, Arend SM et al. Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis. Scand J Immunol 2004;59:66–70 [CrossRef][PubMed]
    [Google Scholar]
  9. Ganguly N, Siddiqui I, Sharma P. Role of M. tuberculosis RD-1 region encoded secretory proteins in protective response and virulence. Tuberculosis 2008;88:510–517 [CrossRef][PubMed]
    [Google Scholar]
  10. van Ingen J, de Zwaan R, Dekhuijzen R, Boeree M, van Soolingen D. Region of difference 1 in nontuberculous Mycobacterium species adds a phylogenetic and taxonomical character. J Bacteriol 2009;191:5865–5867 [CrossRef][PubMed]
    [Google Scholar]
  11. Gcebe N, Rutten V, Gey van Pittius NC, Michel A. Prevalence and distribution of non-tuberculous mycobacteria (NTM) in cattle, African buffaloes (Syncerus caffer) and their environments in South Africa. Transbound Emerg Dis 2013;60:74–84 [CrossRef][PubMed]
    [Google Scholar]
  12. Turenne CY, Tschetter L, Wolfe J, Kabani A. Necessity of quality-controlled 16S rRNA gene sequence databases: identifying nontuberculous Mycobacterium species. J Clin Microbiol 2001;39:3637–3648 [CrossRef][PubMed]
    [Google Scholar]
  13. Telenti A, Marchesi F, Balz M, Bally F, Böttger EC et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175–178[PubMed]
    [Google Scholar]
  14. Adékambi T, Colson P, Drancourt M. rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 2003;41:5699–5708 [CrossRef][PubMed]
    [Google Scholar]
  15. Adékambi T, Berger P, Raoult D, Drancourt M. rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int J Syst Evol Microbiol 2006;56:133–143 [CrossRef][PubMed]
    [Google Scholar]
  16. Adékambi T, Drancourt M. Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int J Syst Evol Microbiol 2004;54:2095–2105 [CrossRef][PubMed]
    [Google Scholar]
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994;22:4673–4680 [CrossRef][PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef][PubMed]
    [Google Scholar]
  19. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012;19:455–477 [CrossRef][PubMed]
    [Google Scholar]
  20. Gurevich A, Saveliev V, Vyahhi N, Tesler G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 2013;29:1072–1075 [CrossRef][PubMed]
    [Google Scholar]
  21. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014;30:2068–2069 [CrossRef][PubMed]
    [Google Scholar]
  22. Wayne LG. Recognition of Mycobacterium fortuitum by means of the 3-day phenolphthalein sulfatase test. Am J Clin Pathol 1961;36:185–197[Crossref]
    [Google Scholar]
  23. Singh P, Wesley C, Jadaun GP, Malonia SK, das R et al. Comparative evaluation of Löwenstein-Jensen proportion method, BacT/ALERT 3D system, and enzymatic pyrazinamidase assay for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 2007;45:76–80 [CrossRef][PubMed]
    [Google Scholar]
  24. Kilburn JO, O'Donnell KF, Silcox VA, David HL. Preparation of a stable mycobacterial tween hydrolysis test substrate. Appl Microbiol 1973;26:836[PubMed]
    [Google Scholar]
  25. Kent PT, Kubica GP. Public Health Mycobacteriology: A Guide for the Level III Laboratory. Atlanta Atlanta, GA: Centres for disease Control, US department of Health and Human Services; 1985
    [Google Scholar]
  26. Brown-Elliott BA, Wallace RJ. Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria. Clin Microbiol Rev 2002;15:716–746 [CrossRef][PubMed]
    [Google Scholar]
  27. Devulder G, Pérouse de Montclos M, Flandrois JP. A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol 2005;55:293–302 [CrossRef][PubMed]
    [Google Scholar]
  28. Gcebe N, Michel A, Gey van Pittius NC, Rutten V. Comparative genomics and proteomic analysis of four non-tuberculous Mycobacterium species and Mycobacterium tuberculosis complex: occurrence of shared immunogenic proteins. Front Microbiol 2016;7:795 [CrossRef][PubMed]
    [Google Scholar]
  29. Tsukamura M, Yano I, Imaeda T. Mycobacterium moriokaense sp. nov., a rapidly growing, nonphotochromogenic Mycobacterium. Int J Syst Bacteriol 1986;36:333–338 [CrossRef]
    [Google Scholar]
  30. Adékambi T, Raoult D, Drancourt M. Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense group species associated with chronic pneumonia. J Clin Microbiol 2006;44:3493–3498 [CrossRef][PubMed]
    [Google Scholar]
  31. Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 2003;16:319–354 [CrossRef][PubMed]
    [Google Scholar]
  32. Shojaei H, Goodfellow M, Magee JG, Freeman R, Gould FK et al. Mycobacterium novocastrense sp. nov., a rapidly growing photochromogenic mycobacterium. Int J Syst Bacteriol 1997;47:1205–1207 [CrossRef][PubMed]
    [Google Scholar]
  33. Turenne C, Chedore P, Wolfe J, Jamieson F, May K et al. Phenotypic and molecular characterization of clinical isolates of Mycobacterium elephantis from human specimens. J Clin Microbiol 2002;40:1230–1236 [CrossRef][PubMed]
    [Google Scholar]
  34. Tortoli E, Rindi L, Bartoloni A, Garzelli C, Manfrin V et al. Isolation of a novel sequevar of Mycobacterium flavescens from the synovial fluid of an AIDS patient. Clin Microbiol Infect 2004;10:1017–1019 [CrossRef][PubMed]
    [Google Scholar]
  35. Bojalil LF, Cerbon J, Trujillo A. Adansonian classification of mycobacteria. J Gen Microbiol 1962;28:333–346 [CrossRef][PubMed]
    [Google Scholar]
  36. Gcebe N, Rutten V, Pittius NGV, Naicker B, Michel A. Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization. Int J Syst Evol Microbiol 2017;67:832–838 [CrossRef][PubMed]
    [Google Scholar]
  37. Crick PJ, Guan XL. Lipid metabolism in mycobacteria–Insights using mass spectrometry-based lipidomics. Biochim Biophys Acta 2016;1861:60–67 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002707
Loading
/content/journal/ijsem/10.1099/ijsem.0.002707
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error