sp. nov., isolated from a squid Free

Abstract

A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136, was isolated from a squid , caught in the East Sea, off Korea. LPB0136 contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol%. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus with an independent genomic species status, showing sequence similarities of 95.9 % to SMK-4 and HJ-26M. The detected respiratory quinone (MK-6) and major polar lipid (phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus . The major cellular fatty acids profile (iso-C, iso-C 3-OH and iso-CG) was also similar to those of members of genus but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus . On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LPB0136 (=KACC 18887=JCM 31564).

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2018-05-01
2024-03-28
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References

  1. Bernardet JF. Family I. Flavobacteriaceae . In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paste BJ et al. (editors) Bergey's Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York: Springer; 2011 pp. 106–111
    [Google Scholar]
  2. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 2001; 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  3. Jung SY, Oh TK, Yoon JH. Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 2006; 56:1577–1581 [View Article][PubMed]
    [Google Scholar]
  4. Yoon JH, Kang SJ, Oh TK. Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 2005; 55:793–798 [View Article][PubMed]
    [Google Scholar]
  5. Shin SK, Kim E, Choi S, Yi H. Cochleicola gelatinilyticus gen. nov., sp. nov., Isolated from a marine gastropod, Reichia luteostoma . J Microbiol Biotechnol 2016; 26:1439–1445 [View Article][PubMed]
    [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  7. Jeon YS, Lee K, Park SC, Kim BS, Cho YJ et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64:689–691 [View Article][PubMed]
    [Google Scholar]
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  9. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A et al. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012; 61:539–542 [View Article][PubMed]
    [Google Scholar]
  10. Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D et al. Toward an online repository of standard operating procedures (SOPs) for (meta)genomic annotation. OMICS 2008; 12:137–141 [View Article][PubMed]
    [Google Scholar]
  11. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  12. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  13. da Costa MS, Albuquerque L, Nobre MF, Wait R. The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. Method Microbiol 2011; 38:197–206 [Crossref]
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  15. da Costa MS, Albuquerque L, Nobre MF, Wait R. The identification of polar lipids in prokaryotes. Method Microbiol 2011; 38:165–181 [Crossref]
    [Google Scholar]
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