1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 68, Issue 5

gen. nov., sp. nov., isolated from seawater Free

Abstract

A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated MM-14, was isolated from seawater sampled from the Yellow Sea in the Republic of Korea and its taxonomic position was investigated using a polyphasic approach. Strain MM-14 grew optimally at 30 °C and in the presence of approximately 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain MM-14 clustered with the type strain of . The novel strain exhibited a 16S rRNA gene sequence similarity value of 96.06 % to the type strain of , but higher 16S rRNA gene sequence similarity values (96.13–96.69 %) to the type strains of , and . Strain MM-14 contained MK-6 as the predominant menaquinone and iso-C, iso-C G and iso-C 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA G+C content of strain MM-14 was 34.6 mol%. The phylogenetic and chemotaxonomic data and other phenotypic properties revealed that strain MM-14 constitutes a new genus and species within the family of the phylum , for which the name gen. nov., sp. nov. is proposed. The type strain of is MM-14 (=KACC 19434=KCTC 62139=NBRC 113019).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): novel genus , novel species , polyphasic taxonomy , Pseudobizionia ponticola and seawater
© 2018 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002691
2018-05-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/68/5/1467.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002691&mimeType=html&fmt=ahah

References

  1. Krieg NR, Ludwig W, Euzéby J, Whitman WB. Phylum XIV. Bacteroidetes phy. nov. In Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed. vol. 4 New York: Springer; 2011 pp. 25
     [Google Scholar]
  2. Cottrell MT, Kirchman DL. Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl Environ Microbiol 2000; 66:5116–5122 [View Article][PubMed]
     [Google Scholar]
  3. Kirchman DL. The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 2002; 39:91–100 [View Article][PubMed]
     [Google Scholar]
  4. Jung YT, Yoon SY, Lee JS, Yoon JH. Taeania maliponensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 2016; 66:3552–3557 [View Article][PubMed]
     [Google Scholar]
  5. Yoon JH, Kang CH, Jung YT, Kang SJ. Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm. Int J Syst Evol Microbiol 2013; 63:685–690 [View Article][PubMed]
     [Google Scholar]
  6. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:2969–2974 [View Article][PubMed]
     [Google Scholar]
  7. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  8. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  9. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  10. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993 [Crossref]
     [Google Scholar]
  11. Bernardet JF, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  12. Reichenbach H. The order Cytophagales . In Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH et al. (editors) The Prokaryotes, A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed. New York: Springer; 1992 pp. 3631–3675
     [Google Scholar]
  13. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes Berlin: Springer; 1981 pp. 1302–1331
     [Google Scholar]
  14. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957; 49:25–68 [View Article][PubMed]
     [Google Scholar]
  15. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968; 95:1921–1942[PubMed]
     [Google Scholar]
  16. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46:502–505 [View Article]
     [Google Scholar]
  17. Yoon JH, Lee ST, Kim SB, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47:111–114 [View Article]
     [Google Scholar]
  18. Yoon JH, Kim IG, Shin DY, Kang KH, Park YH. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 2003; 53:53–57 [View Article][PubMed]
     [Google Scholar]
  19. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [Crossref]
     [Google Scholar]
  20. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  21. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  22. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley and Sons; 1994 pp. 121–161
     [Google Scholar]
  23. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  24. Xamxidin M, Wu YH, Jian SL, Zhou YD, Wang CS et al. Aquaticitalea lipolytica gen. nov., sp. nov., isolated from Antarctic seawater. Int J Syst Evol Microbiol 2016; 66:2657–2663 [View Article][PubMed]
     [Google Scholar]
  25. Shakeela Q, Shehzad A, Tang K, Zhang Y, Zhang XH. Ichthyenterobacterium magnum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from olive flounder (Paralichthys olivaceus). Int J Syst Evol Microbiol 2015; 65:1186–1192 [View Article][PubMed]
     [Google Scholar]
  26. Park S, Lee JS, Lee KC, Yoon JH. Formosa undariae sp. nov., isolated from a reservoir containing the brown algae Undaria pinnatifida . Int J Syst Evol Microbiol 2013; 63:4130–4135 [View Article][PubMed]
     [Google Scholar]
  27. Liu C, Zhang XY, Wen XR, Shi M, Chen XL et al. Arcticiflavibacter luteus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from intertidal sand. Int J Syst Evol Microbiol 2016; 66:144–149 [View Article][PubMed]
     [Google Scholar]
  28. Park S, Lee JS, Lee KC, Yoon JH. Geojedonia litorea gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from coastal seawater. Antonie van Leeuwenhoek 2013; 103:1255–1262 [View Article][PubMed]
     [Google Scholar]
  29. Lee YM, Hwang CY, Lee I, Jung YJ, Cho Y et al. Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix . Antonie van Leeuwenhoek 2014; 106:527–533 [View Article][PubMed]
     [Google Scholar]
  30. Nedashkovskaya OI, Vancanneyt M, Kim SB. Bizionia echini sp. nov., isolated from a sea urchin. Int J Syst Evol Microbiol 2010; 60:928–931 [View Article][PubMed]
     [Google Scholar]
  31. Song EJ, Lee MH, Seo MJ, Yim KJ, Hyun DW et al. Bizionia psychrotolerans sp. nov., a psychrophilic bacterium isolated from the intestine of a sea cucumber (Apostichopus japonicus). Antonie van Leeuwenhoek 2014; 106:837–844 [View Article][PubMed]
     [Google Scholar]
  32. Hameed A, Shahina M, Lai WA, Lin SY, Liu YC et al. Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014. Int J Syst Evol Microbiol 2015; 65:336–345 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002691
Loading
Pseudobizionia ponticola gen. nov., sp. nov., isolated from seawater
Int J Syst Evol Microbiol 68, 1467 (2018); https://doi.org/10.1099/ijsem.0.002691
/content/journal/ijsem/10.1099/ijsem.0.002691
/content/journal/ijsem/10.1099/ijsem.0.002691
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error